聚乳酸-羟基乙酸编织网／胶原-壳聚糖真皮替代物的设计及生物学性能初步评价  

Design and biological evaluation of poly.lactic-co-glycolic acid （PLGA） mesh/collagen-chitosan hybrid scaffold （CCS） as a dermal substitute

作　　者：王新刚[1] 有传刚[1] 孙华凤[1] 胡信雷[1] 韩春茂[1] 张莉萍[1] 郑玉蓉[1] 李齐寅[1] 

机构地区：[1]浙江大学医学院附属第二医院烧伤科,杭州310009

出　　处：《中华烧伤杂志》2011年第1期16-20,共5页Chinese Journal of Burns

基　　金：基金项目：国家重点基础研究发展计划（2005CB623902）;浙江省重大科技专项（2007C13040）

摘　　要：目的设计并构建一种含有编织网的连续性真皮再生模板，并初步评价其生物学性能。方法采用冷冻-冻干法将聚乳酸-羟基乙酸（PLGA）编织网整合到胶原-壳聚糖支架（CCS）中，形成PLGA编织网／胶原-壳聚糖复合支架（PCCS），对比PLGA编织网、PCCS、CCS微观形态及机械强度。将PCCS及CCS分别埋植于18只SD大鼠皮下（PCCS组、CCS组，每组9只大鼠）。术后1、2、4周取埋植部位皮肤标本，行组织形态学观察及免疫组织化学观察（用CD68抗体对巨噬细胞染色），蛋白质印迹法检测CD68、髓过氧化物酶（MPO）、IL-1B、IL—10的蛋白含量。对数据行单因素方差分析和t检验。结果含有编织网的PCCS具有类似CCS的三维多孔结构；PLGA编织网与PCCS的机械强度分别为（3．07±0．10）、（3．26±0．15）MPa，明显优于CCS[（0．42±0．21）MPa，F=592．3，P〈0．0001]。PCCS组约在术后2周完成全层组织长入，CCS组于术后4周才实现这一过程。PCCS组、CCS组均出现巨噬细胞聚集的现象，在术后2周达到高峰，且CCS组可见更多数量的巨噬细胞浸润。术后2周，PCCS组IL-10蛋白含量高于CCS组，而CD68、MPO、IL-1β蛋白含量则低于CCS组（t=-4．06—2．89，P〈0．05或P〈0．01）。结论支架的三维结构与机械强度之间的相互作用，在诱导组织再生中非常重要。PCCS连续性真皮再生模板具有优良的机械性能，合适的三维多孔结构和快速诱导组织、细胞及血管长人的潜能，作为真皮替代物具有一定应用前景。Objective To design and construct and to preliminarily evaluate its biological characteristics a kind of dermal regeneration template with mesh, Methods PLGA mesh was integrated into CCS with freeze-drying method for constructing PLGA mesh/CCS composite （PCCS）. The micromorphologies and mechanical properties among PLGA mesh, CCS, and PCCS were compared. PCCS and CCS was respectively implanted into subcutaneous tissue of SD rats （ PCCS and CCS groups, 9 rats in each group）. The tissue samples were collected at post operation week （POW） 1 , 2, and 4 for histopathological and immunohistochemical observation. Protein levels of CD68, MPO, IL-I β, IL-10 were examined by Western blot, with expression of gray value. Data were processed with one-way analysis of variance and t test. Results Threedimensional porous structure of PCCS was similar to that of CCS. Mechanical property of PLGA mesh and PCCS was respectively （3.07 ± 0. 10）, （3. 26 ± 0. 15） MPa, and they were higher than that of CCS [（0.42±0.21） MPa, F =592.3, P 〈0.0001）]. The scaffolds were filled with newly formed tissue in PCCS group at POW 2, while those in CCS group were observed at POW 4. A large accumulation of macrophages was observed in both groups, especially at POW 2, and more macrophage infiltration was observed in CCS group. The protein level of IL-10 in PCCS group at POW 2 was obviously higher than that in CCS group, while the protein levels of CD68, MPO, IL-1β were significantly decreased as compared with those in CCSgroup （ with t value from - 4. 06 to 2. 89 , P 〈0.05 or P 〈0.01）. Conclusions PCCShas excellent mechanical property with appropriate three-dimensional porous structure. Meanwhile, it can rapidly induce formation of new tissue and vascularization, and it has a prospect of serving as a dermal substitute.

关 键 词：再生 聚乳酸-羟基乙酸 胶原-壳聚糖 

分 类 号：R318.08[医药卫生—生物医学工程]