脂联素受体在人肾上腺皮髓质及其肿瘤中的不同表达  

The mRNA expression of adiponectin receptor 1 and 2 in human adrenal tissues and tumors

作　　者：陈适[1] 曾正陪[1] 李汉忠[2] 童安莉[1] 卢琳[1] 宋爱羚[1] 李明[1] 

机构地区：[1]中国医学科学院北京协和医学院北京协和医院内分泌科卫生部内分泌重点实验室,北京100730 [2]中国医学科学院北京协和医学院北京协和医院泌尿外科,北京100730

出　　处：《基础医学与临床》2011年第2期134-138,共5页Basic and Clinical Medicine

基　　金：国家科技十五攻关计划(2004BA720A29)

摘　　要：目的观察脂联素受体1(AdipoR1)、脂联素受体2(AdipoR2)在正常肾上腺皮、髓质及其肿瘤中的mRNA表达。方法提取6例正常人肾上腺皮质和髓质、10例皮质醇分泌瘤、14例醛固酮分泌瘤和20例嗜铬细胞瘤组织的总RNA,用RT-PCR方法检测AdipoR1、AdipoR2 mRNA表达;并分析AdipoR2与AdipoR1的关系。31例患者曾行3 h葡萄糖耐量试验(OGTT),比较AdipoR1/R2与患者OGTT时血糖和胰岛素曲线下面积(GLUAUC、INSAUC)间的关系。结果在肾上腺皮、髓质及其肿瘤中存在AdipoR1、AdipoR2的mRNA表达。AdipoR1 mRNA在正常肾上腺皮质内的表达明显低于皮质醇分泌瘤和醛固酮分泌瘤(P<0.01),AdipoR1 mRNA在嗜铬细胞瘤内的表达明显高于正常肾上腺髓质(P<0.01)。AdipoR2 mRNA在正常肾上腺皮质内的表达明显低于皮质醇分泌瘤(P<0.05)。皮质醇分泌瘤内AdipoR2 mRNA表达明显高于醛固酮分泌瘤(P<0.05)。在所有组织中,AdipoR2与AdipoR1 mRNA表达高度相关(r=0.335,P<0.01),AdipoR2 mRNA表达与GLUAUC呈正相关(r=0.633,P<0.001)。结论肾上腺皮髓质及其肿瘤广泛表达脂联素受体并存在差异,可能与不同的脂联素水平相关。Objective To compare the mRNA expression of adiponectin receptor 1 and adiponectin receptor 2 （Adipo R1/R2） in human adrenal tissues and tumors. Methods Total RNA was exracted from 6 normal human adrenal glands, 10 cortisol-secreting tumors, 20 pheochromacytomas and 14 aldosteronomas. We used RT-PCR to compare the mRNA expression of Adipo R1/R2 in human adrenal tissues and tumors and analyzed the relationship between Adipo R1/R2, the GLUAuc and INSAuc of 3 h OGTT. Results The mRNA expressions of AdipoR1/GAPDH and AdipoR2/GAPDH were significantly lower in normal human adrenal cortex than that in aldosteronoma and cortisol-secreting tumors（P 〈0. 01 ）. The mRNA expressions of AdipoR1/GAPDH in pheochromacytomas were higher than that in normal human adrenal medulla（P 〈0. 01 ）. The mRNA expressions of AdipoR2/GAPDH in aldosteronoma were lower than cortisol-secreting tumors（P 〈 0. 05 ）, Adipo R1 positively correlated with Adipo R2 （r = 0. 335, P 〈 0. 01 ）,Adipo R2 positively correlated with GLUAUC （r = 0. 633 ,P 〈 0. 001 ）. Conclusion Adiponectin was expressed in adrenal tissues, the difference may be explained by the plasma adiponectin.

关 键 词：脂联素受体1 脂联素受体2 肾上腺肿瘤 mRNA 

分 类 号：R346[医药卫生—基础医学]