人精浆中性α-1,4-糖苷酶活性与精子透明质酸酶活性的关系  

Relationship among seminal plasma neutral alpha-1,4 glycosidase activity and the positive rate and activity intensity of hyaluronidase in infertile males

作　　者：马晓萍[1] 高晓勤[2] 丁贤胜[1] 倪佳[1] 

机构地区：[1]贵阳医学院组织胚胎学教研室,贵州贵阳550004 [2]遵义医药高等专科学校,贵州遵义563002

出　　处：《基础医学与临床》2011年第2期166-169,共4页Basic and Clinical Medicine

基　　金：贵州省省长专项基金[黔科教办(2007)03];贵州省科技厅攻关项目[黔科合S字(2007)1030];高层次人才科研条件特助经费(TZGF2009-37)

摘　　要：目的探讨人精浆中性α-1,4-糖苷酶(中性α-1,4-G)活性与精子透明质酸酶(HYD)活性(HYD阳性反应率及HYD活性强度)的关系。方法收集年龄24～40岁不育男性精液38例,正常对照12例,根据精浆中性α-1,4-G活性检测结果分为2组:精浆中性α-1,4-G活性正常组(26例)和精浆中性α-1,4-G活性异常组(12例)。24～40岁正常生育男性精液12例作为对照组。用检测盒测定中性α-1,4-G活性,用改良固定底物膜法分别检测精子顶体内HYD阳性反应率及HYD活性强度。结果不育各组中的精浆中性α-1,4-G活性和精子HYD活性(HYD阳性反应率、HYD活性强度)均显著低于正常生育组(P<0.01)。精浆中性α-1,4-G活性与HYD活性(HYD阳性反应率、HYD活性强度)呈显著正相关(r=0.877,r=0.910;P<0.01)。结论精浆中性α-1,4-G活性对精子HYD活性(HYD阳性反应率、HYD活性强度)有明显影响。Objective To study the relationship among neutral alpha-1,4 glycosidase（ α-1,4-G） activity of human seminal plasma and the positive rate and activity intensity of hyaluronidase （HYD）. Methods Thirty eight samples of semen were devided into 2 groups according to the result of neutral α-1,4-G activity test. Normal birth masculine seminal fluid matched ages was the control group （12cases）. Neutral α-1,4-G activity in semen test kits was used to detect the neutral α-1,4-G activity. The positive rate and activity intensity of HYD in 2 groups were examined by our improved fixed-substrate film method. Results There was significant difference between normal birth group and infertile groups（P 〈0. 01 ）. The relation between neutral α-1,4-G activity and the positive rate of HYD showed a significant positive correlation （r = 0. 877 ,P 〈 0. 01 ） ; the relation between neutral α-1,4-G activity and the activity intensity of HYD showed a significant positive correlation （ r = 0. 910, P 〈 0. 01 ）. Conclusion The neutral α-1, 4-G activity of human seminal plasma may affect the positive rate and activity intensity of HYD.

关 键 词：男性不育 精浆中性α-1 4-糖苷酶 透明质酸酶 

分 类 号：R698.2[医药卫生—泌尿科学]