TWEAK促进大鼠心肌成纤维细胞增殖和胶原合成  

TWEAK promotes proliferation and collagen synthesis of rat cardiac fibroblasts

作　　者：陈慧娜[1] 任满意[2] 魏峰涛[1] 卢淑霞[1] 杨兆瑞[1] 徐冬玲[1] 王旭平[3] 隋树建[1] 

机构地区：[1]山东大学第二医院心内科,山东济南250033 [2]山东省胸科医院心内科,山东济南250013 [3]山东大学齐鲁医院心内科教育部和卫生部心血管重构与功能研究重点实验室,山东济南250012

出　　处：《基础医学与临床》2011年第2期170-173,共4页Basic and Clinical Medicine

基　　金：山东省自然科学基金(Y2007C073)

摘　　要：目的探讨肿瘤坏死因子样凋亡微弱诱导剂(TWEAK)对大鼠心肌成纤维细胞(CFs)增殖和胶原合成的影响。方法用胰酶消化法培养新生Wistar大鼠CFs,加入重组人TWEAK(rhTWEAK)干预48 h,采用四甲基偶氮唑蓝(MTT)法测定细胞增殖,qRT-PCR检测Ⅰ型和Ⅲ型胶原mRNA,Western blot检测Ⅰ型胶原蛋白表达。结果 rhTWEAK作用后CFs的细胞数目明显增加,Ⅰ型和Ⅲ型胶原mRNA表达上调,同时Ⅰ型胶原蛋白表达显著增强。结论 TWEAK具有促进大鼠心肌成纤维细胞增殖和胶原合成的作用。Objective To investigate the effects of tumor necrosis factor-like weak inducer of apoptosis （TWEAK） on the proliferation and collagen synthesis of cultured rat cardiac fibroblasts （CFs）. Methods Cultured CFs from neonatal Wistar rats were isolated by trypsinization, treated with recombinant human TWEAK （rhTWEAK）, and examined after 48 h. The CFs proliferation was examined by MTT, and the expressions of collagen I and III mRNA and protein were determined by qRT-PCR. The expression of collagen I protein was determined by Western blot. Results rhTWEAK significantly increased the number of CFs and elevated the expression levels of collagen I and III mRNA and collagen I protein. Conclusion TWEAK can promote proliferation and collagen synthesis of rat CFs.

关 键 词：肿瘤坏死因子样凋亡微弱诱导剂 成纤维细胞生长因子诱导早期反应蛋白14 心肌成纤维细胞 增殖 胶原合成 

分 类 号：R542.23[医药卫生—心血管疾病]