腺苷对豚鼠左心室流出道自律细胞的电生理效应  

Electrophysiological effects of adenosine on pacemaker cells in guinea-pig left ventricular outflow tract

作　　者：赵兰平[1] 李建东[1] 商爱民[2] 牛欣[3] 

机构地区：[1]河北北方学院生理教研室,河北张家口075000 [2]河北北方学院教务处,河北张家口075000 [3]北京中医药大学生理教研室,北京100029

出　　处：《基础医学与临床》2011年第2期174-178,共5页Basic and Clinical Medicine

基　　金：河北省科学技术研究与发展计划(062761880)

摘　　要：目的研究腺苷对豚鼠左心室流出道自律性电活动及其对肾上腺素、低氧和酸中毒电生理效应的影响。方法采用玻璃微电极细胞内电位记录技术,分别观察腺苷对左心室流出道自发慢反应电位及对肾上腺素、低氧和酸中毒所致该电位改变的影响。结果 (1)10、50及100μmol/L腺苷可呈剂量依赖性降低豚鼠左心室流出道自律细胞的电活动。(2)10μmol/L肾上腺素可明显提高左心室流出道的自律性,50μmol/L腺苷明显降低肾上腺素导致的该部位自律性升高。(3)低氧和酸中毒均可使该部位的自律性电活动明显降低,50μmol/L腺苷可使其自律性进一步降低。结论腺苷对左心室流出道的自律性及肾上腺素、低氧和酸中毒导致的自律性异常有一定影响。Objective To investigate the effects of adenosine on spontaneous action potentials of left ventricular outflow tract and the effects of adenosine on epinephrine, hypoxia and acidosis. Methods By using conventional intracellular microelectrode technique to record action potentials, adenosine was used to study the electrophysiological effects on pacemaker cells in ventricular outflow tract. The effects of adenosine on epinephrine, hypoxia and acido- sis were also studied. Results （ 1 ） Adenosine significantly decreased the electric activities of pacemaker cells in guinea pig left ventrieular outflow tract. （2）10 μmol/L epinephrine resulted in a significant increase in the auto- rhythmicity of left ventricular outflow tract. 50 μmol/L adenosine inhibited the effects of epinephrine notably. （3） Hypoxia and acidosis led to a marked decrease in the autorhythmicity of left ventricular outflow tract. 50μmol/L adenosine led to a notably decrease in the effects of hypoxia and acidosis. Conclusion Adenosine may significantly decrease the autorhythmicity of left ventricular outflow tract and alter the effects of epinephrine, hypoxia and acidosis.

关 键 词：腺苷 左心室流出道 自律细胞 电生理效应 

分 类 号：Q463[生物学—生理学] R331.3[医药卫生—人体生理学]