丹参非特异性脂质转移蛋白基因(SmLTP1)的克隆及其表达分析  

Cloning and Expression Analysis of A Non-Specific Lipid Transfer Protein Gene (SmLTP1) from Salvia miltiorrhiza Bunge

作　　者：刘梅[1] 生华[1] 化文平[1] 储君[1] 王喆之[1] 

机构地区：[1]药用资源与天然药物化学教育部重点实验室西北濒危药材资源开发国家工程实验室陕西师范大学生命科学学院,西安710062

出　　处：《植物生理学报》2011年第1期63-68,共6页Plant Physiology Journal

基　　金：国家自然科学基金(31000134);陕西省科技研究发展(攻关)计划(2006K16-G15)

摘　　要：对丹参EST序列进行Blast分析,获得一个新的非特异性脂质转移蛋白基因,命名为SmLTP1(GenBank注册号为EF187461)。该基因cDNA全长593bp,包含一个长为357bp的开放读码框,编码118个氨基酸。生物信息学结构分析表明,该蛋白具有植物nsLTP的典型结构,即4对二硫键,4个α-螺旋,1个可结合和容纳脂肪酸分子的类似口袋状的疏水结构。实时荧光定量PCR分析结果表明,SmLTP1基因在丹参不同组织器官中差异表达,其表达受病原菌和茉莉酸甲酯的诱导,显示SmLTP1基因在植物防御反应中发挥作用。By analyzing EST sequences of Salvia miltiorrhiza, we found a new gene which showed high homology with plant non-specific lipid transfer proteins and named as SmLTP1 （GenBank accession number： EF187461）. SmLTP1 consists of 593 nucleotides, including a single 357-bp opening reading frame and encoding a 118-amino-acid peptide. Bioinformatics analysis showed that SmLTP1 had the typical structure of the plant non-specific lipid transfer protein, including 4 pairs of disulfide, 4 or-helices and 1 tunnel-like hydrophobic cavity which could contain lipid molecules. Quantitative RT-PCR analysis revealed that the gene expressed in different organs, and could be induced by methyl jasmonate （MeJA） and pathogen. These results showed that the SrnLTP1 might be pathogen-responsive gene in plant defenses and involve in a complex gene regulation network.

关 键 词：丹参 非特异性脂质转移蛋白 基因克隆 生物信息学分析 实时荧光定量PCR 

分 类 号：Q943.2[生物学—植物学] S567.53[农业科学—中草药栽培]