侵染番茄的中国番木瓜曲叶病毒基因组结构特征  

Genomic Characterization of Isolate of Papaya Leaf Curl China Virus in Solanum lycopersicon

作　　者：王玉[1,2] 丁铭[2] 杨莉[2] 杨长楷[3] 杨向东[3] 张仲凯[2] 

机构地区：[1]昆明理工大学生命科学与技术学院,云南昆明650224 [2]云南省农业科学院生物技术与种质资源研究所,云南昆明650223 [3]云南省农业科学院热区生态农业研究所,云南元谋651300

出　　处：《西南农业学报》2010年第6期1917-1922,共6页Southwest China Journal of Agricultural Sciences

基　　金：国家自然科学基金地区联合资助项目(30860163;2008GAO28)

摘　　要：从云南元谋采集表现曲叶症状的番茄样品中分离粉虱传双生病毒(Whitefly-transmitted geminivirus,WTGs)分离物YN678。序列分析表明其DNA-A为单链环状,全序列长度为2739 bp,编码6个ORFs。BLAST比对发现,该分离物与双生病毒科菜豆金色黄花叶病毒属的中国番木瓜曲叶病毒HeNZM1分离物(PaLCuCNV-[HeNZM1])最为接近,相似性为98.5%,表明番茄中的分离物YN678是PaLCuCNV的1个分离物。利用WTGs卫星分子DNAβ特异引物Beta01/Beta02进行PCR扩增、克隆和测序,在YN678中扩增到DNAβ分子,全长为1357 bp,该序列与中国番茄黄曲叶病毒YN149分离物(TYLCCNV-[YN149])伴随的DNAβ相似性最高,达到99.1%。这是首次从云南番茄中分离到中国番木瓜曲叶病毒。Begomovirus isolate YN678 was obtained from Solanum lycopersicon plants showing leaf curl symptoms collected in Yuanmou,Yunnan province.Complete sequence of the single circular DNA-A molecule comprised 2739 nucleotides and contains six open reading frames.Comparison and analysis showed that total DNA-A of YN678 was most closely related to Papaya leaf curl China virus（PaLCuCNV） with 98.5 % nucleotide sequences identity isolated from HeNZM1.It was concluded that isolate YN678 infecting Solanum lycopersicon was one of PaLCuCNV isolates.Satellite DNA molecule DNAβ was found to be associated with YN678 using the primers beta01 and beta02.The DNAβ of YN678 contained 1357 nucleotides having the hightest nucleotide sequence identity（99.1 %） with DNAβ associated with TYLCCNV-YN149.This was the first report of Solanum lycopersicon infected with PaLCuCNV in Yunnan.

关 键 词：中国番木瓜曲叶病毒 中国番茄黄曲叶病毒 番茄 DNA-A DNAΒ 

分 类 号：S641.2[农业科学—蔬菜学]