附子种胚培养技术研究  

Research on Embryo Culture Method of Aconitum camichaeli Debx.

作　　者：夏燕莉[1] 胡平[1] 丁建[2] 周先建[1] 张美[1] 郭俊霞[1] 舒光明[1] 

机构地区：[1]四川省中医药科学院,四川成都610041 [2]四川省自然资源科学研究院,四川成都610015

出　　处：《西南农业学报》2010年第6期2167-2170,共4页Southwest China Journal of Agricultural Sciences

基　　金：科技部十五攻关项目(2004BA721A31);四川省育种攻关项目(2006yzgg12-21);四川省科技支撑计划(2009FZ0082);四川省中医药科学院基本科研业务专项(A-2008N-15)

摘　　要：首次开展附子种胚培养技术体系研究。以吸水膨胀的种胚为外植体,筛选获得最佳诱导愈伤组织培养基MS+2,4-D 1mg/L,愈伤组织分化出苗培养基MS+6-BA4 mg/L+NAA0.05 mg/L,增殖培养基MS+6-BA4 mg/L+NAA0.3 mg/L,增殖倍数可达20倍,生根培养基1/2MS+IBA1 mg/L+KT1 mg/L,移栽基质以蛭石∶珍珠岩∶泥炭=1∶1∶1为最佳,成活率可达80%。To study the embryo culture method of Aconitum camichaeli Debx.for the first time.Swollen embryo was taken as explants.The best callus inducing medium was screened out as MS＋2,4-D 1 mg/L.The best seedling differentiation medium was screened out as MS＋6-BA 4 mg/L＋NAA 0.05 mg/L.The best proliferation medium was screened out as MS＋6-BA 4 mg/L＋NAA 0.3 mg/L and the proliferation multiples reached 20.The best rooting medium was screened out as 1/2MS ＋ IBA 1 mg/L ＋ KT 1 mg/L.The best transplanting substrate was screened out as the mixture of vermiculite,perlite and turves with equivalent volume.Survival rate of transplanting culture seedling reached 80 %.

关 键 词：附子 种胚培养 成活 

分 类 号：S565.9[农业科学—作物学]