高锰酸钾-亚硫酸钠-核黄素化学发光体系的研究  

Chemiluminescence System of Potassium Permanganate-Sodium Sulfite-Riboflavin

作　　者：李丽清[1] 周艳梅 陶秀俊[1] 封满良[2] 吕九如[2] 

机构地区：[1]山东泰安师范专科学校化学系,泰安271000 [2]陕西师范大学化学系,西安710062

出　　处：《分析化学》1999年第11期1333-1336,共4页Chinese Journal of Analytical Chemistry

摘　　要：研究了核黄素在酸性条件下与高锰酸钾和亚硫酸钠发生化学发光反应的行为,建立了流动注射化学发光测定核黄素的新方法.核黄素浓度在1.0×10^(-7)～1.0×10^(-5)g/mL范围内与化学发光强度呈良好的线性关系,检出限为3×10^(-8)g/mL.对4.0×10^(-7)g/mL的核黄素进行11次平行测定,得方法的相对标准偏差为1.6%.方法用于药剂中核黄素含量测定,结果与药典标准方法——紫外分光光度法测得值一致.An analytical method consisting of flow-injection sampling and chemiluminescence detection for determination of riboflovin is described. It is based on the chemiluminescence reaction of KMn04 -Na2SO3-riboflavin system. The chemiluminescence intensity was correlated linearly with concentration ofriboflavin in the range of 1.0×10-7～1.0×10-5 g/mL. The relative standard deviation is 1.6% for 11measurements of 4.0×10-7g/mL riboflavin standard solution. The method has been applied to thedetennination of riboflavin in pharmaceutical preparations and the results are in good agreement with those by standard method.

关 键 词：核黄素 化学发光 高锰酸钾 测定 亚硫酸钠 

分 类 号：Q563.203[生物学—生物化学] R977.22[医药卫生—药品]