水溶性凝胶渗透色谱法测定芸芝多糖组分的分子量及其相对含量  

Determination of Molecular Weight and Relative Content of the Components of Polysaccharide Krestin with Aqueous Gel Permeation Chromatography

作　　者：李静[1] 左雄军[1] 

机构地区：[1]中国科学院广州化学研究所纤维素开放实验室,广州510650

出　　处：《分析化学》1999年第8期942-944,共3页Chinese Journal of Analytical Chemistry

基　　金：中国科学院广州分院测试基金;华南理工大学制浆造纸国家重点实验室资助课题

摘　　要：用水溶性凝胶渗透色谱法考察了芸芝多糖在水溶性凝胶柱上的吸附影响因素,建立了最佳分离的测试条件;成功地将芸芝多糖的混合物分离为3个组分,测得各组分的分子量分别为1,190,000、796,000和552,000;各组分相对百分含量分别为83.6%、8.9%、7.5%.Polysaccharide krestin, a mixture of proteoglycan extracted from coriolus versicolor in basidimycete was separated by aqueous gel permeation chromatography. Effects of buffer concentration of sodium nitrate on the separation were investigated. Three ingredients were well separated under an optimized condition of 0. 05 mol/L NaNO3. The molecular weight and relative content of them aremeasured to be: Ⅰ, 1,190,000, 83.6%;Ⅱ,796,000, 8.9% and Ⅲ 552,000, 7.5% respectively.

关 键 词：凝胶渗透色谱 芸芝多糖 分子量 测定 相对含量 

分 类 号：TQ464.1[化学工程—制药化工] O629.12[理学—有机化学]