二硫苏糖醇对刺参卵母细胞体外成熟的影响  

DTT induction of oocyte maturation for sea cucumber Apostichopus japonicus(Slenka)

作　　者：庞振国[1,2] 孙慧玲[3] 常亚青[4] 于佳平[4] 

机构地区：[1]中国科学院海洋研究所,青岛266071 [2]中国科学院研究生院,北京100049 [3]中国水产科学研究院黄海水产研究所,青岛266071 [4]大连海洋大学农业部海洋水产增养殖学重点开放实验室,116023

出　　处：《渔业科学进展》2010年第6期82-89,共8页Progress in Fishery Sciences

基　　金：国家863课题项目(2006AA10A411);国家自然科学基金项目(31072230)共同资助

摘　　要：用海星鞘神经提取液和二硫苏糖醇对刺参卵母细胞进行了体外人工促熟研究。结果显示,二硫苏糖醇对刺参卵母细胞具有明显的促熟作用。刺参卵母细胞包被在滤泡中发育,卵母细胞通过动物极的卵突起连接到单层细胞构成滤泡膜上,充分发育的卵母细胞处于生发泡期,染色质凝集在核仁周围,处于生发泡期的卵母细胞在海水中不会自发成熟。用二硫苏糖醇处理充分发育的卵母细胞20min,卵母细胞的生发泡开始移动,并锚定在卵母细胞的动物极,继而出现生发泡破裂。此后,减数分裂重新启动,第一、二极体分别排出。但海星鞘神经提取液对刺参卵母细胞没有促熟作用。实验表明,二硫苏糖醇浓度在10-5～10-1mol/L范围内,均可促使刺参卵母细胞发生生发泡破裂,10-2mol/L时诱导卵母细胞成熟率可达90%以上。Water-extracts of radial nerves from starfish and dithiothreitol（DTT）were used to induce the oocyte maturation for sea cucumber Apostichopus japonicus（Slenka）.Although radial nerve extracts failed to induce oocyte maturation for sea cucumber,breakdown of germinal vesicles and subsequent meiotic behavior of chromosomes were induced by the treatment with DTT.Oocytes of the sea cucumber A.japonicus were arrested at the germinal vesicle（GV）stage and did not start maturation spontaneously.After being treated with DTT for 20 min,the GV began to migrate to the animal pole of the oocyte,then the GV broke down,which is called germinal vesicle breakdown（GVBD）.Meiosis of the oocyte reinitiated and two polar bodies protruded out successively.DTT with the concentration of 10-1~10-5 mol/L successfully induced GVBD.Maturation percentage reached 90% when the concentration of the DTT solution was 10-2 mol/L.Oocytes induced to maturation could be fertilized,while they could not develop normally.

关 键 词：刺参 卵母细胞 体外促熟 二硫苏糖醇 

分 类 号：S968.9[农业科学—水产养殖]