广东省烟区烟草花叶病主要病原鉴定  

Identification of main pathogeny of tobacco mosaic viruses in Guangdong province

作　　者：杨雷亮[1] 陈泽鹏 邓海滨[3] 陈伟明 李华平[1] 

机构地区：[1]华南农业大学植物病毒研究室,广州510642 [2]广东省烟草公司,广州510610 [3]广东烟草南雄科学研究所,南雄512400

出　　处：《中国烟草学报》2010年第6期72-76,共5页Acta Tabacaria Sinica

基　　金：广东省烟草专卖局科技项目(粤烟科【2003】17号)

摘　　要：为明确广东省烟草病毒病种类及其主要侵染病原,对广东2个主要烟区(韶关地区和梅州地区)进行了病毒病害调查,并对采自田间的62个花叶病样本,通过鉴别寄主、ELISA和RT-PCR法进行了病原种类鉴定。结果表明:在上述2个主要烟区,主要是花叶病危害,很少发现其他病毒种类;病原鉴定方面,在62个花叶病样本中,61.29%为单TMV侵染,20.97%为单CMV侵染,17.74%为TMV和CMV复合侵染。Investigation of viral diseases in tobacco in two main regions（Shaoguan and Meizhou） in Guangdong province was carried out in order to find out pathogeny.62 samples showing mosaic symptoms from fields were collected and analyzed by mechanical inoculation of identified hosts,ELISA,and RT-PCR.Results showed that tobacco plants were mainly infected by mosaic viruses,and rarely by other viruses in the two districts.Identification of the types of mosaic viruses revealed that 61.29% and 20.97% of samples were infected by single virus of TMV and CMV respectively,and 17.74% were infected by both TMV and CMV.

关 键 词：烟草花叶病 病原 鉴定 

分 类 号：S435.72[农业科学—农业昆虫与害虫防治]