异马钱子碱对心肌细胞作用的单钙通道及透射电镜分析  

SINGLE CHANNEL ANALYSIS AND ELECTRON MICROGRAPH STUDY ON MYOCARDIAL EFFECTS OF ISOBRUCINE

作　　者：陆跃鸣[1] 陈龙[1] 蔡宝昌[1] 马骋[1] 史智阳 李显 

机构地区：[1]南京中医药大学,南京210029

出　　处：《安徽中医学院学报》1999年第6期47-49,共3页Journal of Anhui Traditional Chinese Medical College

基　　金：国家自然科学基金;江苏省教委自然科学基金

摘　　要：用膜片钳的方法，在培养ｗｉｓｔａｒ 大鼠乳鼠心室肌细胞上分别记录Ｔ 型、Ｌ 型、Ｂ 型钙通道的单通道活动。异马钱子碱显著激动３ 种钙通道的活动，使其开放时间延长，关闭时间缩短，开放概率增加，而对通过每一种离子通道的离子流幅值无明显影响。采用电镜观察的方法。Single channel activities of T type,L type,and B type Ca 2+ channels were recorded on single ventricular myocardiocyte of wistar rats,using cell attached patch clamp technique.We discovered that isobrucine 50μg/ml excited the activities of the three types of Ca 2+ channels significatively,increased the open state probability and lengthened the open time,without apparent influence on the amplitude of ionic current flow through the Ca 2+ channels.The antioxidative effect of isobrucine were studied by observation under electron microscope of cultured myocardiocytes.The results indicated that X XOD destroyed the myofilaments and mitochondria.Isobrucine was able to antagonize the variations induced by X XOD.

关 键 词：马钱子 心肌细胞 培养 钙通道 心肌 超微结构 

分 类 号：R285.5[医药卫生—中药学]