高温热击处理对培养水稻花药愈伤组织形成的影响  

Effect of Thermal Shock Treatment on the Formation of Rice Anther Callus

作　　者：张跃非[1] 王金玲[2] 邓晓容[1] 

机构地区：[1]绵阳市农业科学研究所,四川绵阳621023 [2]绵阳师范学院,四川绵阳621000

出　　处：《绵阳师范学院学报》2010年第11期81-84,共4页Journal of Mianyang Teachers＇ College

摘　　要：该文对水稻花药进行了不同温度和不同时间处理,然后进行愈伤组织诱导。结果表明:水稻花药在培养前高温热击处理的最适处理温度为35℃,时间以24 h为宜。热处理温度过低时间太短,不利于愈伤组织形成,导致绿苗产量下降。热处理温度过高时间过长,白化苗急剧上升。In this paper,rice anthers are treated by thermal shock at different temperatures for different times and then the callus is induced.The result shows that the optimum thermal shock treatment temperature is 35℃ and the appropriate time is 24h before the rice anther culture.Both low heat-shock temperature and short time are not conducive to the formation of rice callus,which results in the decrease of green seedlings,while high heat treatment temperature and long heat shock leads to sharp increase of albino.

关 键 词：水稻花药培养 愈伤组织诱导率 绿苗分化率 白化苗 

分 类 号：Q813[生物学—生物工程]