金鱼致病菌的分离鉴定与药敏特性  

Isolation,identification and drug sensitivity of pathogen from goldfish

作　　者：曹海鹏[1] 刘丽玲[1] 李怡[1] 杨先乐[1] 

机构地区：[1]上海海洋大学,国家水生动物病原库,上海201306

出　　处：《淡水渔业》2010年第6期46-49,55,共5页Freshwater Fisheries

基　　金：现代农业产业技术体系建设专项资金;农业部水产种质资源与利用重点开放实验室专项(KFT2008-5);上海高校选拔培养优秀青年教师科研专项(SSC-07006);淡水鱼类出血性疾病综合防治技术集成示范(200803013)

摘　　要：采用常规方法对患病金鱼(Carassius auratus)内脏组织(肝脏、肾脏、性腺)中致病菌进行了分离鉴定与药敏特性研究。结果显示:实验共分离了8株优势菌,经人工回归感染实验证实其中3株细菌(菌株LK-T、PL-R和PK-T)具有致病性。ATB细菌鉴定仪鉴定结果显示,菌株LK-T和菌株PL-R为温和气单胞菌(Aeromonas so-bria),菌株PK-T为豚鼠气单胞菌(Aeromonas punctata subsp.caviae)。这3株致病菌的主要特征为:革兰氏染色阴性,杆状,对奈替米星、头孢呋肟、头孢拉啶、庆大霉素、环丙沙星、新霉素、先锋必等药物均高度敏感。其中庆大霉素、环丙沙星、新霉素等常规渔药可作为防治用药的依据。Eight dominant bacteria were isolated from the abodominal organs（liver,kidney and gonad）of the diseased goldfish using the conventional method.Among the eight strains,three bateria,temporarily named LK-T,PL-R and PK-T,were proved to be pathogenic,and were identified by ATB bacteria identification instrument.The results showed that strain LK-T and PL-R were Aeromonas sobria,strain PK-T was Aeromonas punctata subsp.caviae.The three pathogenic bacteria were all gram-negative and rod-shaped.They were highly sensitive to netilmicin,cefuroxime,cefradine,gentamicin,ciprofloxacin,neomycin and cefobid.Therefore,gentamicin,ciprofloxacin,and neomycin could be used for the control of this disease.

关 键 词：金鱼(Carassius auratus) 温和气单胞菌(Aeromonas sobria) 豚鼠气单胞菌(Aeromonas punctata subsp.caviae) 药敏特性 

分 类 号：S941.42[农业科学—水产养殖]