不同种源地桉树抗寒性能的比较  

Comparision on Cold Resistance of Eucalyptus from Different Provenances

作　　者：庄国庆[1] 胡天宇[1] 郭洪英[1] 刘均利[1] 陈炙[1] 杨晓蓉[1] 

机构地区：[1]四川省林业科学院生物技术与良种研究所,四川成都610081

出　　处：《桉树科技》2010年第1期14-20,共7页Eucalypt Science & Technology

基　　金：国家"十一五"林业科技支撑计划;农林动植物育种工程项目"高产优质桉树速生材树种新品种选育"专题(2006BAD01A1504);四川省农作物育种攻关项目林竹新品种选育"桉树良种选育及杂交育种"子专题(2006YZGG-10)

摘　　要：对来自不同种源地的6个不同种共13个桉树样品进行低温胁迫处理,通过电导率法测定它们的最低半致死温度,同时也测定了MDA、总蛋白含量、叶绿素含量、渗透调节物质如脯氨酸和可溶性糖含量等生理指标,结果发现,随胁迫时间的增加,所有样本MDA以及渗透调节物质含量均增加,赤桉增幅最大,铁木桉最小。样本MDA增加的幅度与样本抗寒能力呈负相关,渗透调节物质增加幅度则与其抗寒能力呈正相关。抗寒性较差的样本叶绿素和淀粉含量在胁迫期呈下降趋势,而在抗寒能力较强的样本中,二者与对照比较变化并不明显。通过比较各项指标发现,不仅不同种桉树的抗寒性能有显著差异,不同种源来源的同一种桉树在抗寒性能上也存在较明显差异。测试的13个不同种源地的桉树抗寒性能由高到低依次是:铁木桉(种源号:151199)>巨桉实生苗>巨桉EG5(组培苗)>迪恩桉(种源号:14521)>邓恩桉(种源号:15967)>邓恩桉(种源号:14113)>邓恩桉(种源号:15956)>邓恩桉(种源号:14452)>卵叶桉(种源号:17285)>卵叶桉(种源号:13745)>边沁桉(种源号:88080)>赤桉(种源号:11911)>赤桉(种源号:15027)。13 eucalyptus samples from different provenances were treated with low-temperature stress ,and their semi-lethal temperature,chlorophyll content,MDA,total protein content,and osmotic adjustment material such as proline and soluble sugar content were determined.The results showed that with the stress time increasing,MDA as well as osmoregulation substances had increased in all the samples. Cold resistance of samples was negatively correlated with the level of MDA,the rate of increase in osmoregulation substances was positively correlated with their cold resistance. During stress period the contents of chlorophyll and protein in lower cold-tolerance samples decreased,but those of in higher cold-tolerance samples didn’t change obviously by comparision with the control. As shown in the results above,there were significant differences not only among different eucalyptus species but between the same species from different provenances in cold resistance. Among 13 eucalyptus species,the cold resistance from strong to weak were：E.sideroxylon（No. 151199）E.grandis（by seed ）E.grandis （by tissue culture）E.deanei （No.14521）E.dunnii（No.15967）E.dunnii（No.14113）E.dunnii（No.15956）E.dunnii（No.14452）E.ovata（No.17285）E.ovata（No.13745）E.benthamii（No.88080）E.camaldulensis（No.11911）E.camaldulensis（No.15027）.

关 键 词：电导率法 桉树 渗透调节物质 抗寒性能 

分 类 号：S792.39[农业科学—林木遗传育种]