史密斯桉组织培养技术研究  

Study on Tissue Culture of Eucalyptus smithii

作　　者：管朝旭 黄键 曾德贤 朱仁刚 范林元 陈剑英 

机构地区：[1]云南省林木种苗工作总站,云南昆明650215

出　　处：《桉树科技》2010年第2期12-17,共6页Eucalypt Science & Technology

摘　　要：本文探讨了史密斯桉组织培养技术。试验表明,经HgCl2消毒后用1%的氯化钙浸洗处理,能有效控制外植体褐化的发生,褐化死亡率仅为12.5%;始芽萌动以及初期继代培养阶段,采用F+6-BA 0.5 mg.L-1+IBA 0.3mg.L-1培养基,有利于外植体的不定芽诱导以及初期的继代扩大培养,增殖系数达3.7;在增殖与伸长生长阶段,采用改良H+6-BA 0.125～0.25 mg.L-1+IBA 0.25 mg.L-1+VC 20 mg.L-1+VB210 mg.L-1培养基,在保证较高增值系数的情况下,有利于芽的伸长生长;以1/3 MS为基本培养基,添加0.25 mg.L-1IBA对史密斯桉的根系诱导较为有效,生根率达37.8%。The technology of Eucalyptus smithii tissue culture was discussed.The experiment showed that the treatment effective to prevent browning was with dipping them into 1% CaCl2 after treated with HgCl2.The rate of brown and mortality rate was only 12.5%.The medium of F＋6-BA 0.5 mg·L-1＋IBA 0.3 mg·L-1 could promote the induction of adventitious buds and multiplication in the initial budding culture and the preliminary stage of subcultures,the ratio of multiplication could reach 3.7.A large ratio could gain and elongation growth of buds could be stimulated by using the medium of modified H＋6-BA 0.125～0.25 mg·L-1＋IBA 0.25 mg·L-1＋ VC 20 mg·L-1 ＋ VB 210 mg·L-1.The medium of 1/3 MS ＋ 0.25 mg·L-1 IBA was suitable for rooting induction,and rooting ratio could be 37.8%.

关 键 词：史密斯桉 组织培养 伸长生长 

分 类 号：S722.37[农业科学—林木遗传育种]