反相高效液相色谱法测定大花红景天及其混淆品中红景天苷的含量  

Determination of salidroside in Rhodiola crenulata and its counterfeits by RP-HPLC

作　　者：史伟[1,2] 陈胜璜[2] 杨大坚[1] Joyce H O 李顺旭[1,2] 

机构地区：[1]香港理工大学深圳研究院,广东深圳518057 [2]湖南中医药大学药学院,长沙410208

出　　处：《中南药学》2010年第12期892-895,共4页Central South Pharmacy

基　　金：香港赛马会慈善信托基金(JCICM-5-07)

摘　　要：目的建立RP-HPLC测定市场上大花红景天及其混淆品中红景天苷含量的方法。方法色谱柱为Phe-nomenex C18(250 mm×4.60 mm,5μm),流动相为甲醇-水(15∶85),等度洗脱,流速为1.0 mL.min-1,检测波长为223 nm,柱温为25℃。结果红景天苷在62.5～1 000μg.mL-1与峰面积线性关系良好(r=0.999 7,n=5),平均加样回收率为100.1%,RSD为0.40%(n=6)。结论本方法简便、准确、重复性强,可用于市面上大花红景天及其混淆品中红景天苷的含量测定。Objective To establish an RP-HPLC method to determine salidroside in Rhodiola crenulata and its confused species in the market. Methods HPLC was used to determine the content of salidroside. The Phenomenex C18 column （250 mm×4. 60 mm, 5um） was applied with methanol-water （15 ： 85） as the mobile phase. The flow rate was 1.0 mL ·min-1. The detection wavelength was 223 nm, and the column temperatrue was 25 ℃. Results The linear range of salidroside was 62.5--1 000ug · mL-1 , and the average recovery was 100.1% （n =6）. Conclusion This is a simple and accurate method, which can be used for the determination of salidroside in Rhodiola crenulata and its confused species.

关 键 词：大花红景天 红景天苷 反相高效液相色谱法 

分 类 号：R927.2[医药卫生—药学] R284.1