脂联素在糖尿病心肌病心肌间质纤维化中的作用机制研究  

Mechanism of Adiponectin on Myocardial Interstitial Fibrosis Reduction in Diabetic Cardiomyopathy

作　　者：张伟[1] 崔彦[1] 于琼兰[1] 

机构地区：[1]深圳市孙逸仙心血管医院,广东深圳518020

出　　处：《实用预防医学》2010年第12期2356-2358,共3页Practical Preventive Medicine

基　　金：2009年深圳市科技计划项目(编号200903138)

摘　　要：目的探讨脂联素(adiponectin,APN)在糖尿病心肌病大鼠心肌间质纤维化中的抑制作用及其实现机制。方法取72只6周龄的清洁级雄性Wistar大鼠,随机分为对照组、实验组、模型组,每组24只,实验前所有大鼠正常喂养1周后测体重和血糖、尿糖。模型组、实验组分别给予空腹一次性注射链脲佐菌素(1%STZ,55 mg/kg)制备糖尿病模型,对照组腹腔注射同体积枸橼酸-枸橼酸钠缓冲液,72 h后模型组、实验组大鼠尾静脉采血测血糖>16.7 mol/L;尿糖(+++^++++),且具有体重减轻,多饮、多食、多尿现象为糖尿病成膜。造模后实验组(APN组)、模型组(Saline组)每天分别给予腹腔注射APN、Saline,持续至造模后第16周末。分别于8、12、16周末空腹麻醉取材评定糖尿病发病程度,测定心脏超声评估心功能;取心脏病理切片进行Masson染色拍片,扫描,采用图像分析仪定量心肌组织胶原的含量以评定心肌纤维化情况;心脏病理切片进行DHE染色以评估氧化应激情况。结果 Saline组至16周末死亡5只,发生糖尿病大鼠为17只(89.5%);而APN组无死亡,7只发生糖尿病(29.2%)。与Saline组大鼠相比,APN组大鼠左心室舒张末期内径(LVEDD)、左心室收缩末期内径(LVESD)、左心室舒张末期容积(LVEDV)和左心室收缩末期容积(LVESV)均显著减少(P<0.05),短轴缩短率(FS)和射血分数(EF)显著升高(P<0.05),左室胶原容量分数(CVF)显著减少(P<0.05),反映氧化应激情况的DHE染色相对发光值显著减弱(P<0.05)。结论早期的APN干预治疗能够通过抗氧化应激效应降低糖尿病心肌病大鼠心肌纤维化。Objective To explore the effect of adiponectin（APN） on myocardial interstitial fibrosis reduction in diabetic cardiomyopathy rats and to analyze its mechanism. Methods Seventy-two clean degree male Wister rats were selected and divided into control group,experimental group（APN group） and model group（Saline group） randomly,each group had 24 rats.Before the experiment,body weight,blood glucose and urine glucose of all the rats were detected at the end of one week feeding in common.The rats in experimental group and model group were given a single intraperitoneal injection of streptozotocin（1% STZ,55 mg/kg） to prepare the diabetic models.The rats in control group were given the same volume intraperitoneal injection of citral-sodium citrate buffer.72 hours later,the rats of experimental group and model group whose blood glucose was higher than 16.7 mol/L,urine glucose（＋＋＋-＋＋＋＋） and who had polydipsia,hyperphagia and polyuria were considered to be diabetic rats.The rats in experimental group and model group were given intraperitoneal injection of APN and saline separately and continued until the 16th week post-STZ.The severity degree of diabetic in rats was assessed at the end of 8,12,and 16 weeks,and then cardiac function was evaluated by ultrasound.Heart biopsy was taken to conduct Masson＇s staining film and scan,image quantitative analysis was used to detect myocardial collagen content in order to assess myocardial fibrosis.The oxidative stress situation was evaluated by DHE staining. Results After 16 weeks treatment intervention,5 rats in saline group died,but no death occurred in APN group.17（89.5%） rats in saline group developed diabetic,but there were only 7（29.2%） rats in APN group developed diabetic.As compared with saline group,LVEDD,LVESD,LVEDV and LVESV were significantly reduced（P〈0.05）,FS and EF were significantly elevated（P〈0.05）,left ventricular collagen volume fraction（CVF） and relative luminescence values of DHE staining reduced significantl

关 键 词：脂联素 糖尿病心肌病 心肌纤维化 氧化应激 

分 类 号：R541.8[医药卫生—心血管疾病]