转TaDREB3基因大豆的农艺性状研究  

Study on agronomic traits of transgenic soybean overexpressing TaDREB3

作　　者：张彬彬[1] 刘淼[1] 高冬梅[1] 马有志[2] 李文滨[1] 

机构地区：[1]东北农业大学大豆生物学教育部重点实验室,哈尔滨150030 [2]中国农业科学院作物科学研究所,北京100081

出　　处：《东北农业大学学报》2010年第12期6-10,共5页Journal of Northeast Agricultural University

基　　金：转基因生物新品种培育专项(2008zx08004-002;2009zx08011-023B;2009zx08009-089B)

摘　　要：将抗逆基因TaDREB3转入东农50,得到稳定遗传株系,并将T4代株系种子播于盐碱地上。将其生育期、株高、主茎节数、分枝数、单株荚数、单株粒数、百粒重等性状和对照东农50进行比较,研究在较高盐碱条件下该基因对大豆农艺性状方面的影响。结果表明,在较高盐碱条件下,转基因株系和对照植株相比,生育期并没有改变,而主茎节数、单株荚数和分枝数等农艺性状呈显著或极显著提高,这表明转TaDREB3基因大豆对盐碱胁迫具有一定的抗性。Dongnong50 was transformed with TaDREB3,a stress-tolerance gene,and transgenic progenies with TaDREB3 showing stable heredity characters were obtained in this study.The seeds of T4 generation were sowed on saline land of which the salt concentration was 0.19% and pH was 8.43,and agronomic traits composed of the growth period,plant height,numbers of main nodes,branching number,pods per plant,and the number of seeds per plant,and the weight of 100-seed were collected and comparisons to control plants Dongnong50 were made.The impacts of gene TaDREB3 on agronomic traits of soybeans under high concentration of the salt were discussed.The results showed that while the growth period was not altered in transgenic line with TaDREB3 under high concentration saline and alkali,the extremely significant or significant increase in the number of main nodes,pods per plant and in branching number and other agronomic traits in transgenic lines in comparison with non-transgenic lines was an indicative of their resistance to saline-alkali stress.

关 键 词：DREB 转基因大豆 抗逆 生育期 农艺性状 

分 类 号：S767.5[农业科学—森林保护学] X172[农业科学—林学]