广西汉坦病毒全S基因核苷酸序列测定  

Study on complete S gene nucletide sequence of Hantavirus in Guangxi province

作　　者：闭福银[1] 谭毅[1] 韦增良[1] 杨进业[1] 

机构地区：[1]广西壮族自治区疾病预防控制中心,南宁530028

出　　处：《应用预防医学》2010年第6期325-328,共4页Applied Preventive Medicine

基　　金：广西壮族自治区卫生厅重点科研课题(重200537)

摘　　要：目的查明广西汉坦病毒的基因型及其基因亚型。方法用夹夜法捕获宿主动物,用免疫吸附试验(ELISA)检测鼠肺标本,对汉坦病毒抗原阳性标本用RT-PCR法扩增,产物直接测序,拼接后获得全S基因序列,用生物软件进行核苷酸及氨基酸系列分析和病毒的系统进化分析。结果应用ELISA法检测鼠肺抗原792份,阳性6份,RT-PCR测定其中5毒株,基因型为汉城病毒,进一步的核苷酸序列分析均为S2亚型。结论广西汉坦病毒基因型为汉城型汉坦病毒,亚型为S2。Objective To investigate the genetypes and sub-types of Hantavirus in Guangxi province.Methods Host animals were collected and Hantavirus in the lung tissues were identified by Enzyme-Linked Immunosorbent Assay（ELISA）.The complete S segments of positive samples were amplified with RT-PCR and sequenced.Phylogenetic analysis was used for genotyping.Results 792 specimens of lung tissues were detected by ELISA,and 6 were positive.Five of six positive specimens were detected by RT-PCR and sequenced,and all Hantaviruses were Seoul virus.Phylogenetic analysis of complete S segments showed that Seoul Hantaviruses in Guangxi province were the S2 sub-genotype,and were closely related with the strain from Zhejiang province.Conclusion The genotype of Hantaviruses in Guangxi province was SEO,and the sub-genotype was S2.

关 键 词：汉坦病毒 基因亚型 核苷酸序列 

分 类 号：R373.3[医药卫生—病原生物学]