天然海鱼共附生放线菌抑菌和抗病毒活性筛选研究  

Screening of Marine Fish-derived Actinomycetes Against Pathogenic Fungi and Virus

作　　者：谭庆伟[1,2] 杜丹超[2] 欧阳明安[1,2] 

机构地区：[1]福建农林大学植物病毒研究所 [2]福建农林大学生物农药与化学生物学教育部重点实验室,福建福州350002

出　　处：《激光生物学报》2010年第6期824-831,共8页Acta Laser Biology Sinica

基　　金：福建省自然科学基金(2009J05049;2009N0016);教育部博士点基金(20093515120005)

摘　　要：从台湾海峡天然海鱼共附生微生物群落分离得到29株放线菌,通过抗病毒和抑菌活性筛选,分别得到6株对烟草花叶病毒和植物病原真菌具有较强抑制作用的天然菌株。其中,菌株030603对供试植物病原真菌拮抗作用尤为显著,其发酵产物具有广谱抑菌活性,可以作为广谱性植物病原真菌拮抗菌株进行生防菌产业化开发及发酵产物活性成分的化学研究。该菌株形态特征、培养特性、生理生化特性与微白黄链霉菌基本一致,16 S rDNA序列同源性达99.5%。实验结果表明,利用天然海洋鱼类共附生微生物群落获取植物病原菌拮抗菌株具有取样便捷和活性菌株检出率高的特点。Twenty-nine actinomycetes were isolated from symbiotic and epiphytic micro-organisms of natural marine fi-shes, among which six strains showed potent antagonistic activities against all of the four pathogenic fungi used for screening, including Rhizoctonia cerealis, Fusarium oxyspoum, Pythium aphanidermatum and Alternaria solani, while another six strains showed moderate inhibition ：against the replication of Tobacco mosaic virus strain 030603, which was i- dentified as Streptomyces albidoflavus by morphology, cultural characteristics, physiological and biochemical properties, chemotaxonomy and 16S rDNA sequences study, possessed a broad-spectrum antimicrobial activity. The screening results revealed that marine fish-derived aetinomycetes may be a good means of convenient and effective approach used for study of marine micro-organism.

关 键 词：放线菌 天然海鱼 植物病原真菌 烟草花叶病毒 

分 类 号：S432.4[农业科学—植物病理学]