看麦娘对稀禾啶和高效氟吡甲禾灵产生抗药性的分子基础  

The Molecular Basis of Resisitance to Sethoxydim and Haloxyfop-R-methyl in Alopecurus aequalis Sobol.

作　　者：黄世霞[1,2] 何金铃[1] 王庆亚[2] 张守栋[2] 刘学诗[1] 

机构地区：[1]安徽农业大学生命科学学院,安徽合肥230036 [2]南京农业大学生命科学学院,江苏南京210095

出　　处：《激光生物学报》2010年第6期832-837,共6页Acta Laser Biology Sinica

基　　金：江苏省十五攻关项目(BE2001346);安徽省高等学校优秀青年人才基金(2011)

摘　　要：采用双向等位基因特异性PCR研究不同抗药性生物型看麦娘(Alopecurus aequalis Sobol.)对乙酰辅酶A羧化酶(ACCase)抑制剂类除草剂稀禾啶(sethoxydim)和高效氟吡甲禾灵(haloxyfop-R-methyl)产生交互抗性的分子基础。结果表明:对稀禾啶产生的高抗性的JXRII生物型能特异性多扩增出约495 bp的特异片断,而对稀禾啶表现敏感性及中等抗性生物型只能扩增出约770 bp的片断,比对序列发现编码的氨基酸由亮氨酸(Leu)替代了异亮氨酸(Ile)。在研究其对高效氟吡甲禾灵产生抗药性分于基础时发现对该除草剂产生高抗型LYR生物型能特异性多扩增出约490 bp的片断,对其表现敏感性及中等抗性生物型只能扩增出1 100 bp,进一步证实了靶标酶ACCase可能存在多个突变位点而产生不同模式的抗药性,同时也表明芳氧苯氧基丙酸类(AOPP)和环己烯酮类(CHD)除草剂的作用位点是有差异的。The bi-directional allele-specific PCR technology was used to detect the molecular basis of resistance to such herbicide like sethoxydim and haloxyfop-R-methyl, which can inhibit the activity of acetyl-CoA carboxylase （ACCase） , in Alopecurus aequalis Sobol. The results showed that the highly sethoxydim-resistant biotype （JXRII） could be amplified an additional 495 bp-specific fragment, while either susceptible biotype or moderately resistant biotype amplified a 770 bp fragment only. After sequencing, it was discovered that an ACCase mutant allele was resided in the genome of Alopecurus aequalis Sobol. , with a base T instead of A at nucleotide 5341. For amino acids sequence, it suggested that a leucine（Leu） residue was substituted with an isoleucine（Ile）. The basis in molecular level of the resistance to haloxyfop- R-methyl in Alopecurus aequalis Sobol. was also studied, highly resistant biotype （LYR） could be amplified an additional 490 bp-specific fragment, while others could only be amplified a 1 100 bp fragment. It was revealed that there was another ACCase mutation at nucleotide 6 278, suggesting a multiple-mutation phenomenon in this case. At the same time, It also illuminated the target sites were different between aryloxyphenoxypropionate （AOPP） and eyclohexanedione （CHD）.

关 键 词：看麦娘 等位基因特异性PCR 乙酰辅酶A羧化酶 抗药性 

分 类 号：S481[农业科学—农药学]