新疆结核分枝杆菌临床分离株Spoligotyping基因型的初步研究  

Spoligotyping of clinical isolates of Mycobacterium tuberculosis in Xinjiang

作　　者：綦迎成 刘洁[2] 鱼栓民 李君莲 赵秀芹[2] 王泉 刘志广[2] 吕冰[2] 刘中华[2] 万康林[2] 

机构地区：[1]新疆自治区胸科医院,新疆乌鲁木齐830000 [2]中国疾病预防控制中心传染病预防控制所/传染病预防控制国家重点实验室,北京102206 [3]新疆克孜勒苏人民医院

出　　处：《疾病监测》2010年第12期951-954,共4页Disease Surveillance

基　　金：国家自然科学基金"中国结核分枝杆菌基因多态性及株水平鉴定技术研究"(No.30771853);国家"十一五"重大传染病防治科技重大专项"结核病传播模式研究结核病分子流行病学研究"(No.2008ZX100/03-010-02)~~

摘　　要：目的研究新疆结核分枝杆菌Spoligotyping基因分型,初步了解其基因型多态性状况及主要流行株。方法在新疆维吾尔自治区胸科医院收集一个连续时间段的结核分枝杆菌临床分离菌株,采用比例法检测进行耐药性检测、间隔寡核苷酸分型(Spoligotyp ing)方法进行分型研究。基因聚类分析采用B ioNum erics 5.0数据库软件,统计学分析采用χ2检验,P<0.05为差异有统计学意义。结果共收集到结核分枝杆菌临床分离菌株175株,其中对利福平、异烟肼、链霉素和乙胺丁醇全敏感115株,耐药60株(包括单耐药31株和耐多药29株)。经Spoligotyp ing分型,这些菌株可分为4个基因群49种基因型,最大的1个基因群为北京家族,占68.57%(120/175)。北京家族中,敏感菌株63.87%(76/119),耐药菌株占36.13%(43/119);非北京家族中,敏感菌株68.52%(37/54),耐药菌株占31.48%(17/54),北京家族与非北京家族的耐药率之间差异无统计学意义(P=0.551)。结论新疆结核分枝杆菌临床菌株存在明显的基因多态性,主要流行菌株为北京基因型。北京基因型与耐药性无明显相关性。Objective To investigate the spoligotyping of the clinical isolates of Mycobacterium tuberculosis in Xinjiang,the gene polymorphism and main prevalent strain of M.tuberculosis in this region.MethodsM.tuberculosis isolates were collected consecutively in The Chest Hospital of Xinjiang Uyghur Autonomous Region.The drug susceptibility test was conducted with proportion method.Genotyping on these strains was conducted with the spacer oligonucleotide typing（Spoligotyping）,and clustering of gene was analyzed with BioNumerics 5.0 software.ResultsA total of 175 M.tuberculosis isolates have been collected,of which 115 were sensitive to four first line drugs： rifampin（RFP）,isoniazid（INH）,streptomycin（SM） and ethambutol（EMB）,60 were drug resistant,including 31 single drug resistant and 29 multi drug resistant strains.The spoligotyping results showed that there were 4 clusters including 49 genotypes,in which the biggest cluster was Beijing strains（Beijing genotype）,accounting for 68.57%（120/175）.Of 119 Beijing strains,76 were drug sensitive and 43 were drug resistant,accounting for 63.87% and 36.13% respectively,while of 54 non-Beijing strains,37 were drug sensitive and 17 were drug resistant,accounting for 68.52% and 31.48% respectively.No significant difference on drug resistance was found between Beijing strains and non-Beijing strains.Conclusion It is confirmed that there is obvious gene polymorphism of clinical strains of M.tuberculosis isolated in Xinjiang,the main prevalent strain belongs Beijing genotype.The result showed that there is no relationship between Beijing genotype and drug resistance.

关 键 词：结核分枝杆菌 基因分型 间隔寡核苷酸分型 北京基因型 

分 类 号：R378[医药卫生—病原生物学]