生物絮凝剂生产菌的分离与鉴定及其絮凝特性分析  

Isolation and Identification of Bioflocculant Producing Stain and Analysis on Its Flocculating Characteristics

作　　者：马龙[1] 刘伟杰[1] 李宝珍[1] 袁红莉[1] 杨金水[1] 

机构地区：[1]中国农业大学生物学院农业部农业微生物资源及其应用重点实验室,北京100193

出　　处：《安徽农业科学》2010年第35期19882-19884,19900,共4页Journal of Anhui Agricultural Sciences

基　　金：研究生科研创新专项(2009);国家自然科学基金项目(30700010);国家公益性行业农业专项(200803033)

摘　　要：[目的]对分离自土壤样品中的一株微生物絮凝剂产生菌W5进行系统鉴定并分析其絮凝特性。[方法]以细菌的16S rDNA通用引物对其基因组进行PCR扩增,并采用单因素法分析培养基的pH值、碳源和氮源对絮凝活性的影响,采用红外光谱法进行功能基团的分析。[结果]同源性比对结果表明,菌株W5与Hymenobacter gelipurpurascens具有98.34%的同源性,为革兰氏阴性,长杆菌。菌株W5生长发酵培养基最佳条件为pH值7.04、葡萄糖为碳源、酵母粉为氮源。对菌株W5所产的微生物絮凝剂(MBF-W5)进行提取纯化,所得产物微红色絮状物。红外分析结果表明,MBF-W5中主要的功能基团为羟基、羧基和氨基。[结论]在絮凝剂用量为150～500μl,温度为5～65℃,pH值为2～10的范围内均具有85%以上的絮凝活性。[Objective] The aims was to indentify a bioflocculatnt producing strain W5 isolated from soil samples and analyze its flocculating characteristics.[Method] The universal primers of bacterial 16S rDNA were used for PCR amplification of its genome;effects of the medium pH,carbon and nitrogen sources on its flocculating activity were analyzed by using single factor method;and infrared spectroscopy was used for functional groups analysis.[Result] The results of homology comparison showed that W5 strain and Hymenobacter gelipurpurascens had homology of 98.34%,belonging to Gram-negative,long bacilli.In addition,the optimum carbon source,nitrogen source,pH value for fermentation condition were glucose,yeast extract and 7.04,respectively.Microbe flocculant produced by W5 strain（MBF-W5） was extracted and purified to obtain reddish and flocculent products.Fourier-transform infrared（FTIR） spectroscopy indicated that the major functional groups of MBF-W5 were hydroxyl,carboxyl and amino.[Conclusion] Flocculating activity more than 85% was achieved under the conditions of bioflocculation dosage of 150-500 μl,temperature of 5-65 ℃ and pH 2-10.

关 键 词：Hymenobacter gelipurpurascens W5 微生物絮凝剂 MBF-W5 絮凝活性 

分 类 号：S183[农业科学—农业基础科学]