一品红组织培养条件优化  

Optimization of Tissue Culture Conditions of Euphorbia pulcherrima

作　　者：李春苗[1] 李鹤春[1] 孙彩玉[1] 孙梅青[1] 王丽娟[1] 

机构地区：[1]东北农业大学生命科学学院,黑龙江哈尔滨150030

出　　处：《安徽农业科学》2010年第35期19918-19921,共4页Journal of Anhui Agricultural Sciences

摘　　要：[目的]为培育出更优良的一品红,实现工厂化育苗。[方法]采用组织培养的方式,通过正交试验优化一品红培养基中植物生长调节物质(PGR)的浓度及配比。[结果]最佳外植体为绿色叶片,最佳培养基为MS培养基,最佳消毒时间7～9 min,初期诱导最佳碳源为3%蔗糖;诱导嫩叶产生愈伤组织的最佳培养基为MS+1.00 mg/L6-BA+0.10 mg/LNAA+0.50 mg/L2,4-D;愈伤组织分化的最佳培养基为MS+1.50 mg/L6-BA+0.10 mg/LNAA+1.50 mg/L2,4-D;丛生芽增殖的最佳培养基为MS+1.00 mg/L6-BA+0.10 mg/LNAA+0.50 mg/L2,4-D;诱导生根最佳培养基为MS+0.05 mg/LNAA。[结论]筛选出最佳的一品红培养基及其最佳PGR配比,为实现一品红工厂化育苗奠定了基础。[Objective]To obtain the better Euphorbia pulcherrima seedlings,so as to culture the seedlings industrially.[Method] By employing the tissue culture,the concentration and formula of plant growth regulator in the medium of Euphorbia pulcherrima culture were optimized by orthogonal test.[Result]The optimal explant and medium were green leaf and MS medium respectively.The optimum disinfecting duration and carbon source were 7-9 min and 3% saccharose respectively.The optimum induction and differentiation medium were confirmed to beMS ＋1.00 mg/L 6-BA ＋ 0.10 mg/L NAA ＋0.50 mg/L 2,4-D and MS＋1.50 mg/L 6-BA ＋0.10 mg/L NAA ＋1.50 mg/L 2,4-D respectively.In addition,the optimum proliferation medium of cluster buds and rooting induction medium were MS＋1.00 mg/L 6-BA ＋0.10 mg/L NAA ＋0.50 mg/L 2,4-D and MS＋0.05 mg/L NAA respectively.[Conclusion] The optimal medium and formula of PGR are screened out in this study,which provides basis for the industrialized cultivation of more favorable Euphorbia pulcherrima.

关 键 词：一品红 最佳外植体 最佳消毒时间 最佳碳源 最佳培养基 

分 类 号：S681.4[农业科学—观赏园艺]