线粒体基因高变区应用于香蕉种属亲缘关系的鉴定研究  

Application of Mitochondria DNA Hypervariable Region in the Identification of Relative Relationships in Musa paradisiaca

作　　者：闻盼盼[1] 郭泽锋[1] 许林兵[2] 黄秉智[2] 董方园[1] 赵景阳[1] 陈谷[1] 

机构地区：[1]华南理工大学轻工与食品学院,广东广州510641 [2]广东省农业科学院果树研究所,广东广州510640

出　　处：《安徽农业科学》2010年第35期19951-19953,共3页Journal of Anhui Agricultural Sciences

摘　　要：[目的]建立一种较为简单有效的香蕉种属关系鉴定的手段和方法。[方法]根据不同香蕉品种线粒体基因内含子序列间的差异性,通过PCR扩增线粒体基因组中细胞色素氧化酶亚基Ⅱ基因中的1个内含子,并进行测序和聚类分析,对分属5个基因型(AAA、AA、AAB、ABB和BB)的16个香蕉品种进行了分类。[结果]16个香蕉品种可分为3大类:第1类包括1个品种,基因型为BB;第2类包括7个品种,基因型为ABB;第3类包括8个品种,基因型包括AA、AAA、AAB和BB。其中,抗病新株系逸仙1、2、3号与粉杂有最近的亲缘关系。[结论]该方法对香蕉品种的分类结果与基因型的组成类型基本一致,说明它在鉴定香蕉种属亲缘关系方面是可行的。[Objective] The aim was to establish an effective method for the identification of relative relationships in Musa paradisaca.[Method] Based on the diversity of mitochondria DNA intron sequence among different varieties of M.paradisaca,an intron of cytochrome oxidase subunit II gene in mitochondria DNA genome was amplified and sequenced.And then the cluster analysis was used to classify 16 varieties of M.paradisaca,which belonged to five genotypes（AAA,AA,AAB,ABB and BB）.[Result] The 16 varieties of M.paradisaca could be divided to three classes： the first class contained one variety,the genotype of which was BB;the second class contained seven varieties,the genotype of which was ABB;the third class contained eight varieties,the genotypes of which included AA,AAA,AAB and BB.The new clones YiXian 1,2 and 3 showed the nearest relationship with FenZa.[Conclusion] The result of classification was consistent with the genotypes,thus verified the feasibility and effectiveness of the new method in the family relationship identification of M.paradisaca germplasm.

关 键 词：香蕉 线粒体基因 内含子 聚类分析 

分 类 号：S668.1[农业科学—果树学]