低浓度医用臭氧对胎鼠离体脊髓神经元钙通道的影响  

Effect of low-concentration ozone on calcium channels in fetal rat spinal cord neurons in vitro

作　　者：林小雯[1] 傅志俭[1] 赵序利[1] 谢珺田[1] 

机构地区：[1]山东大学附属省立医院疼痛科,济南市250021

出　　处：《中华麻醉学杂志》2010年第10期1189-1191,共3页Chinese Journal of Anesthesiology

基　　金：国家自然科学基金（30972844）;山东省自然科学基金（Y2006C69,ZR2010H1097）

摘　　要：目的 探讨低浓度医用臭氧对胎鼠离体脊髓神经元钙通道的影响.方法 原代胎鼠脊髓神经元,接种于培养皿内,随机分为3组,O3-15组（n=4）和O3-20组（n=8）分别暴露于15、20μg/ml医用臭氧中,对照组（C组,n=5）暴露于空气中,20 min后采用全细胞膜片钳技术记录细胞膜钙通道的电生理活动.结果 与C组比较,03-15组与O3-20组峰值钙电流密度升高,钙电流半数激活电压向超极化方向移动（P＜0.05）;O3-20组峰值钙电流密度高于O3-15组（P＜0.05）.3组钙电流稳态失活曲线的半数失活电压差异无统计学意义（P＞0.05）.结论低浓度医用臭氧可使胎鼠离体脊髓神经元钙通道易于激活,其效应与浓度有关.Objective To investigete the effect of low-concentration ozone （O3） on voltage-gated calcium channels in fetal rat spinal cord neurons in vitro. Methods The spinal cord neurons were cultured in vitro and identified by immunohistochemistry staining. Then the cells were seeded into the culture dish and randomly divided into 3 groups： control group （group C, n = 5）, O3 15 μg/ml （group O3-15, n = 4） and O3 20 μg/ml group （group O3-20, n = 8）. The cells were exposed to O3 15 and 20 μg/ml in group O3-15 and O3-20 respectively, while the cells were exposed to air in group C. After 20 min incubation, the electrophysiological activity of calcium channels in neurons was recorded using whole-cell patch-clamp technique. Results Peak calcium current density was significantly increased in group O3-15 and O3-20 compared with group C （ P 〈 0.05）. Half-activation voltage was significantly lower in group O3-15 and O3-20 than in group C （ P 〈 0.05）. Peak calcium current density was significantly higher in group O3 -20 than in group O3-15 （ P 〈 0.05） . There was no significant difference in half-inactivation voltage among the three groups （ P 〉 0.05）. Conclusion Low concentration of O3 can make the activation of the calcium ion channel easy in fetal rat spinal cord neurons in vitro.

关 键 词：臭氧 钙通道 脊髓 神经元 

分 类 号：R285.5[医药卫生—中药学]