糖尿病神经病理性痛大鼠脊髓背角γ-氨基丁酸B1受体表达的变化  

Expression of GABAB1 receptors in spinal dorsal horn neurons in rats with diabetic neuropathic pain

作　　者：刘彦涛[1] 王秀丽[1] 王倩[1] 董蕊[1] 马江红[1] 王秋筠[1] 郭跃先[2] 

机构地区：[1]河北医科大学第三医院麻醉科,石家庄市050051 [2]河北医科大学第三医院泌尿外科,石家庄市050051

出　　处：《中华麻醉学杂志》2010年第10期1216-1219,共4页Chinese Journal of Anesthesiology

基　　金：国家自然科学基金（30772082,30972832）;河北省自然科学基金（C2008000931）;河北省卫生厅资助课题（07090）

摘　　要：目的 探讨糖尿病神经病理性痛（DNP）大鼠脊髓背角γ-氨基丁酸B1（GABAB1）受体表达的变化.方法 SD雄性大鼠60只,随机分为2组（n=30）,DNP组（D组）腹腔注射链脲佐菌素50 mg/kg制备糖尿病模型,正常对照组（C组）给予等容量生理盐水.分别于给予链脲佐菌素或生理盐水后3、5、7周时取10只大鼠,采集静脉血样,测定空腹血糖浓度,然后测定机械缩足阈值,取脊髓组织,分别采用免疫组化法和Western blot法测定脊髓背角GABAB1受体表达水平,采用RT-PCR法测定脊髓背角GABAB1受体mRNA表达水平.结果 与C组比较,D组血糖升高,机械缩足阈值降低,GABAB1受体及GABAB1受体mRNA表达下调（P＜0.05）.结论 DNP大鼠脊髓背角GABAB1受体表达下调.Objective To investigate the role of GABAB1 receptors in spinal dorsal horn neurons in the development of diabetic neuropathic pain （DNP）. Methods Sixty pathogen free male SD rats aged 4 weeks weighing 150-170 g were randomly divided into 2 groups （ n = 30 each）： control group and DNP group. Diabetes mellitus was induced by single intraperitoneal injection of streptozotocin 50 mg/kg. Blood glucose levels and paw withdrawal threshold to mechanical stimuli were measured at 3, 5 and 7 weeks （T1, T2, T3 ） after IP STZ/NS （ n = 10 each）. The animals were sacrificed after PWL measurement. The lumbar segment of the spinal cord was removed for determination of the expression of GABAB1 receptors by immuno-histochemistry, RT-PCR and Western blot analysis. Results The blood glucose levels were significantly higher while the PWT was significantly lower at T1,T2 and T3 in group DNP than in control group. The expression of GABAB1 receptor mRNA and protein in spinal dorsal horn was significantly lower at T2 and T3 in DNP group than in control group. Conclusion The expression of GABAb1 receptors is down-regulated in spinal dorsal horn neurons in rats with DNP.

关 键 词：神经痛 糖尿病并发症 脊髓 受体 GABA-B 

分 类 号：R587.2[医药卫生—内分泌]