七氟醚或缺血预处理对大鼠肺缺血再灌注时ERK和CaM表达的影响  

Effects of sevoflurane or ischemic preconditioning on the expression of ERK and CaM during lung ischemia-reperfusion in rats

作　　者：穆蕊[1] 于泳浩[1] 张加艳[1] 张素品[2] 

机构地区：[1]天津医科大学总医院麻醉科,300052 [2]天津医科大学总医院ICU,300052

出　　处：《中华麻醉学杂志》2010年第10期1261-1263,共3页Chinese Journal of Anesthesiology

摘　　要：目的 探讨七氟醚或缺血预处理对大鼠肺缺血再灌注时细胞外信号调节蛋白激酶（ERK）和钙调素（CaM）表达的影响.方法 健康雄性SD大鼠24只,体重270～320 g,随机分为4组（n=6）：假手术组（S组）、肺缺血再灌注组（IR组）、缺血预处理组（IP组）和七氟醚预处理组（SP组）.IR组采用夹闭左肺门45 min恢复灌注120 min的方法制备肺缺血再灌注损伤模型,IP组缺血前夹闭左肺门缺血5 min恢复灌注5 min,连续2次,SP组缺血前吸人2.1%七氟醚30 min.于再灌注120 min时取左肺组织,测定TNF-α和IL-6含量、ERK mRNA和CaM mRNA的表达水平.结果 与S组比较,IR组、IP组和SP组肺组织TNF-α和IL-6的含量、ERK mRNA和CaM mRNA的表达水平升高（P＜0.05）;与IR组比较,IP组和SP组肺组织TNF-α和IL-6的含量和CaM mRNA的表达水平降低,ERK mRNA表达水平升高（P＜0.05）;SP组和IP组各指标比较差异无统计学意义（P＞0.05）.结论七氟醚预处理和缺血预处理均通过下调CaM表达和上调ERK表达减轻大鼠肺缺血再灌注损伤.Objectlve To investigate the effects of sevoflurane or ischemic preconditioning on the expression of extracellular signal-regulated kinase （ERK） and calmodulin （CaM） during lung ischemia-reperfusion （I/R）in rats. Methods Twenty-four male SD rats weighing 270-320 g were randomly divided into 4 groups （ n = 6each）： sham operation group （group S）, group I/R, ischemic preconditioning group （group IP）, and sevoflurane preconditioning group （group SP）. In group S, the hilum of the left lung was dissociated after thoracotomy but not occluded. In group I/R, lung I/R was produced by occlusion of the hilum of the left lung for 45 min followed by 120 min of reperfusion. In group IP, the hilum of the left lung was occluded for 5 min and unclamped for 5 min for 2 times before the model was established. In group SP, sevoflurane was inhaled for 30 min at the end-tidal concentration of 2.1% before lung ischemia. All rats were sacrificed at 120 min of reperfusion and the lung tissues were taken for determination of the contents of TNF-α and IL-6 by ELISA and the expression of ERK mRNA and CaM mRNA by RT-PCR. Results Compared with group S, the contents of TNF-α and IL-6 and the expression of ERK mRNA and CaM mRNA were significantly increased in group I/R, IP and SP （ P 〈 0.05）. Compared with group I/R, the contents of TNF-α and IL-6 and the CaM mRNA expression were significantly decreased, while the expression of ERK mRNA was significantly increased in group IP and SP （ P 〈 0.05）. There was no significant difference in the each index metioned above between group IP and SP （ P 〉 0.05 ）. Conclusion Sevoflurane preconditioning and ischemic preconditioning can protect the lung from I/R injury through down-regulating the expression of CaM and up-regulating the expression of ERK.

关 键 词：麻醉药 吸入 缺血预处理 细胞外信号调节MAP激酶类 钙-钙调素依赖性蛋白激酶类 再灌注损伤 肺 

分 类 号：R965[医药卫生—药理学]