氧化低分子肝素-抗凝血酶复合物涂层聚氯乙烯管道的制备及抗凝血性能研究  

Preparation and Antithrombogenicity of Oxidated Low Molecular Weight Heparin-Antithrombin Complex Coated-Polyvinyl Chloride Tubing

作　　者：罗鹏[1] 刘维永[1] 杨春[1] 周华[2] 曹瑞军[2] 杨剑[1] 

机构地区：[1]第四军医大学西京医院心血管外科,西安710033 [2]西安交通大学应用化学系,西安710049

出　　处：《生物医学工程学杂志》2011年第1期90-94,共5页Journal of Biomedical Engineering

基　　金：国家自然科学基金资助项目(30700174)

摘　　要：利用蛋白非酶糖基化反应合成NaIO4氧化低分子肝素—抗凝血酶共价复合物(SPLMWATH),采用聚乙烯亚胺-戊二醛结合技术制备SPLMWATH涂层、肝素涂层和低分子肝素涂层聚氯乙烯(PVC)管道,并通过分光光度法和动态凝血时间实验分别测定SPLMWATH合成率以及不同涂层管道的涂层结合密度、涂层脱落率和抗凝血性能。结果表明,SPLMWATH合成率约为55%,与肝素涂层和低分子肝素涂层相比,虽然SPLMWATH涂层PVC管道的涂层结合密度较小,但其稳定性和抗凝血性能均明显优于肝素涂层和低分子肝素涂层。Based on non-enzymatic protein glycated reaction,the sodium periodate-oxidated low molecular weight heparin-antithrombin covalent complex（SPLMWATH） was produced.By using polyethyleneimine-glutaraldehyde bonding technique,polyvinyl chloride（PVC） tubings were coated with SPLMWATH,heparin and low molecular weight heparin（LMWH）.Spectrophotometry and dynamic clotting time experiment were used to determine the synthetic ratio of SPLMWATH,graft density,coating leaching ratio and to evaluate the antithrombogenicity of different coating on the PVC tubings.The results showed that the synthetic ratio of SPLMWATH was approximately 55%,and compared with heparin coating and LMWH coating,the graft density of SPLMWATH coating on the PVC tubing was smaller,but its coating stability and antithrombogenicity were significantly better than that of heparin coating and LMWH coating on the PVC tubings.

关 键 词：氧化低分子肝素-抗凝血酶共价复合物 聚氯乙烯 抗凝血性能 

分 类 号：R318.08[医药卫生—生物医学工程] R654.1[医药卫生—基础医学]