聚乳酸-羟基乙酸(PLGA)网架生物学性能的初步评价  

Preliminary Evaluation of the Biological Properties of Poly-lactide-co-glycolic acid (PLGA) Knitted Mesh

作　　者：于玮洁[1] 王新刚[1] 胡信雷[1] 孙华凤[1] 韩春茂[1] 

机构地区：[1]浙江大学医学院附属第二医院烧伤科,杭州310009

出　　处：《生物医学工程学杂志》2011年第1期163-169,共7页Journal of Biomedical Engineering

基　　金：国家重大基础研究计划资助项目(2005CB623902);浙江省重大专项基金资助项目(2007C13040)

摘　　要：初步探索聚乳酸-羟基乙酸(poly-lactide-co-glycolic acid,PLGA)网架的可降解性、生物相容性、力学性能及其在组织工程领域的应用。综合运用体外降解实验、急性细胞毒性实验、皮内刺激实验和埋植实验对网架的生物学性能进行表征。研究显示,该网架安全无毒,其降解液pH值在6.68～7.33的范围内波动,且降解过程中弹性模量呈先升高后降低的趋势;埋植网架的降解与新生组织的长入同步发生,且网架周围的ED-1+细胞数量随着该网架体内的转归呈先升高后降低的趋势。PLGA网架具有良好的机械性能、生物相容性和降解速率,可作为强化胶原基真皮替代物机械性能的"骨架"。This is a work aimed to investigate the biodegradability,biocompatibility and mechanical property of the poly-lactide-co-glycolic acid（PLGA） knitted mesh preliminarily and to further explore its applications in tissue engineering and regenerative medicine.The biological property of PLGA mesh was investigated comprehensively with the degradation experiment in vitro,the acute cytotoxicity assay,the intradermal irritation test and the subcutaneous implantation test in vivo utilized.The degradation experiment in vitro demonstrated that the pH value of the removed solution fluctuated between 6.68 and 7.33.The elastic modulus of the PLGA mesh increased at first and then decreased afterwards.The acute toxicity test and the intradermal irritation test indicated that the PLGA mesh was with innocuity safety.The PLGA mesh accelerated degradation and was replaced gradually by the neotissue.The results of immunohistochemical staining demonstrated that the number of ED-1＋ cells increased at first and then decreased afterwards.The PLGA mesh with excellent mechanical properties,good biocompatibility and favorable degradation ratio has the potential to be employed as a ＂skeleton＂ to reinforce the mechanical property of collagen-based dermal substitutes in tissue engineering.

关 键 词：网架 生物相容性 真皮替代物 组织工程 

分 类 号：R318.08[医药卫生—生物医学工程]