朝藿定C在不同血浆中蛋白结合率的研究  

Protein binding rates of epimedin C in different plasma samples

作　　者：刘敏彦[1,2] 赵韶华[2] 许红辉[2] 贾继明[2] 李向军[2] 张兰桐[1] 

机构地区：[1]河北医科大学药学院药物分析教研室,河北050017 [2]石家庄以岭药业股份有限公司

出　　处：《北京中医药大学学报》2010年第12期821-824,共4页Journal of Beijing University of Traditional Chinese Medicine

基　　金：国家科技重大专项课题(No.2009ZX09313-003;No.2009ZX09102-140)

摘　　要：目的建立测定朝藿定C在健康人血浆和犬血浆中蛋白结合率的方法。方法采用平衡透析法结合HPLC法对朝藿定C与健康人血浆和犬血浆的蛋白结合率进行测定。采用Waters C18柱,柱温为25℃,流动相为乙腈-水(30∶70),流速为1 mL/min,检测波长为269 nm。结果朝藿定C在不同介质中的精密度和回收率均较好,与人血浆在100、50、25 mg/L质量浓度下的血浆蛋白结合率分别为(75.6±1.6)%、(72.5±1.3)%、(76.5±0.8)%;与犬血浆在100、50、25 mg/L质量浓度下的血浆蛋白结合率分别为(70.0±1.7)%、(72.4±2.2)%、(74.0±2.0)%。结论体外朝藿定C与人血浆和犬血浆蛋白结合率较高,且与血药无明显的浓度依赖性。Objective To establish a method for determination of protein binding rates of epimedin C in human plasma samples and dog plasma samples.Method The protein binding rates of epimedin C in human plasma samples and dog plasma samples were determined by applying equilibrium dialysis combined with HPLC.The HPLC procedure was performed on Waters C18 column at 25 ℃,and the mobile phase was acetonitrile-water（30∶ 70） in the gradient elution.The flow velocity was 1 mL/min and the detection wavelength was 269 nm.Result Epimedin C had higher precision and recovery in different media.The protein binding rates of epimedin C were,respectively,（75.6±1.6）%,（72.5±1.3）% and（76.5±0.8）% in human plasma samples in the mass concentration of 100 mg/L,50 mg/L and 25 mg/L,while in dog plasma samples in the same mass concentration,the protein binding rates were,respectively,（70.0±1.7）%（72.4±2.2）% and（74.0±2.0）%.Conclusion Epimedin C has higher protein binding rates in human plasma samples and dog plasma samples in vitro without significant concentration dependence.

关 键 词：朝藿定C 蛋白结合率 平衡透析法 高效液相色谱 

分 类 号：R285.5[医药卫生—中药学]