消银解毒饮对银屑病血热证外周血淋巴细胞中IL-4、IFN-γ的调控作用  

Regulative effect of Xiaoyinjiedu Decoction on secretion of IL-4 and IFN-γ by peripheral lymphocytes in blood-heat syndrome of psoriasis

作　　者：夏梦[1] 段行武[1] 瞿幸[1] 张云壁[1] 高永红[1] 孙逸坤[1] 

机构地区：[1]北京中医药大学东直门医院,北京100700

出　　处：《北京中医药大学学报》2010年第12期837-840,共4页Journal of Beijing University of Traditional Chinese Medicine

基　　金：国家中医药管理局课题(No.06-07LP05)

摘　　要：目的研究消银解毒饮对银屑病血热证外周血淋巴细胞中白细胞介素-4(IL-4)、γ-干扰素(IFN-γ)的调控作用。方法采用随机对照原则,将入选银屑病血热证患者与正常健康人分别抽取静脉血,分离其外周血淋巴细胞,并与人永生化角质形成细胞混合接种培养24 h。加入5种不同组别动物药理血清,分为蒸馏水组、消银解毒饮组、凉血方组、解毒方组、甲氨蝶呤组培养48 h。用四唑盐比色实验测定药物对人永生化角质形成细胞增殖率,双抗体夹心法测定药物对细胞分泌IL-4、IFN-γ的影响。结果消银解毒饮能调控外周血淋巴细胞分泌IL-4(P<0.05)升高和IFN-γ(P<0.05)下降,从而抑制角质形成细胞的活化增殖,诱导细胞凋亡,且效果优于单组中药,其中凉血类药可能在促进淋巴细胞分泌IL-4方面起主要作用,解毒类药在抑制淋巴细胞分泌IFN-γ方面起主要作用。结论消银解毒饮可能通过对银屑病血热证外周血淋巴细胞中某些细胞因子如IL-4、IFN-γ的免疫调节来达到抑制角质细胞增殖的作用。Objiective To study the regulative effect of Xiaoyinjiedu Decoction on the secretion of IL-4 and IFN-γ by regulation lymphocytes in blood-heat syndrome of psoriasis.Methods The patients with bolld-heat syndrome of psoriasis and normal health cases were chosen based on randomized and controlling principle and the blood samples were collected.The peripheral lymphocytes were separated and given combined inoculation with human immortalized keratinocytes,and then cultured for 24 hours.The animal pathological serum with different volume fractions were added into grouped lymphocyte samples and cultured for 48 hours.The effect of Chinese medicinal on the proliferation of human immortalized keratinocytes was detected by using MTT,and the influence of Chinese medicinal on the secretion of IL-4 and IFN-γ by using double antibody sandwich method.Results Xiaoyinjiedu Decoction regulated the increase of IL-4（P0.05） and decrease of IFN-γ（P0.05） secreted by peripheral lymphocytes,so inhibited the activation and proliferation,and induced the apoptosis of human immortalized keratinocytes.The effect of Xiaoyinjiedu Decoction was better than that of single kind of Chinese medicinal.The medicinal with the action of cooling blood played the major role in improving lymphocytes to secret IL-4,and the medicinal with the action of detoxifying played the major role in inhibiting lymphocytes to secret IFN-γ.Conclusion Xiaoyinjiedu Decoction may inhibit the proliferation of keratinocytes through immuno-regulating IL-4 and IFN-γ secreted by peripheral lymphocytes in blood-heat syndrome of psoriasis.

关 键 词：银屑病 外周血淋巴细胞 人永生化角质形成细胞 细胞因子 消银解毒饮 

分 类 号：R285.5[医药卫生—中药学]