RP-HPLC法测定蒙药复方述达格-4中高良姜素和山奈素的含量  

Quantitative determination of galangin and kaempferide in Shudage-4(Mongolian medicinal formula) by RP-HPLC

作　　者：马强[1,2] 贾鑫[1] 董玉[1] 

机构地区：[1]内蒙古医学院药学院,010059 [2]内蒙古科技厅

出　　处：《北京中医药大学学报》2010年第12期845-847,共3页Journal of Beijing University of Traditional Chinese Medicine

基　　金：2008年内蒙古自治区科技计划项目(No.20080502);2009年内蒙古自治区重大科技计划项目(No.2009-0020959);2010年内蒙古自治区高等学校科学研究项目(No.NJ10137);2010年呼和浩特市科技计划项目(No.2010-农-社-3)

摘　　要：目的建立同时测定蒙药复方述达格-4中高良姜素、山奈素的含量测定方法。方法采用反相高效液相色谱(RP-HPLC)法,色谱柱为Diamosil C18(4.6 mm×250 mm,5μm),以乙腈(A)-0.5%磷酸水溶液(B)为流动相,梯度洗脱(0～20 min,34%A→37%A,20～60 min,37%A)。流速1 mL/min,检测波长为286 nm,柱温30℃,进样量20μL。结果高良姜素、山奈素的线性范围分别为0.04～0.40μg(r=0.999 9)、0.016 56～0.165 60μg(r=0.999 9),平均加样回收率(n=6)分别为98.21%、98.93%,RSD分别为1.77%、2.55%。结论本法准确、简便,具有良好的重复性,可为蒙药复方述达格-4质量控制提供依据。Objective To establish a method for quantitative determination of galangin and kaempferide in Shudage-4（Mongolian medicinal formula） by RP-HPLC at the same time.Methods The RP-HPLC procedure was performed on the chromatographic column of Diamosil C18（4.6 mm×250 mm,5 μm）,and the mobile phase was acetonitrile（A）-0.5 % phosphoric acid solution in gradient elution（0-20 min,34% A to 37% A;20-60 min,37% A）.The flow velocity was 1 mL/min,detection wavelength was 286 nm and temperature was 30 ℃.The added quantity of samples was 20 μL.Results The range of linear relationship of galangin and kaempferide was,respectively,at 0.04-0.40 μg（r=0.999 9）,0.016 56-0.165 60 μg（r=0.999 9）.The average recovery（n=6） was,respectively,98.21%（RSD=1.77%） and 98.93%（RSD=2.55%）.Conclusion The method was accurate and simple with a higher repeatability,which can be used for the quality control of Shudage-4.

关 键 词：高效液相色谱法 蒙药述达格-4 高良姜素 山奈素 含量测定 

分 类 号：R284.2[医药卫生—中药学]