髓鞘结构在听神经瘤组织中的检测  

Detect myelin structure in acoustic tumor

作　　者：王琰[1] 姜海洋[1] 于何[1] 关超[1] 姜学钧[1] 

机构地区：[1]中国医科大学附属第一医院耳鼻咽喉科,沈阳110001

出　　处：《临床耳鼻咽喉头颈外科杂志》2011年第4期169-171,175,共4页Journal of Clinical Otorhinolaryngology Head And Neck Surgery

基　　金：国家自然科学基金科学部主任基金资助(No:30540080)

摘　　要：目的:探讨来源于施万细胞的人类听神经细胞瘤组织中是否存在髓鞘结构及其细胞学特点。方法:应用免疫荧光标记、Western blotting及电镜等方法检测听神经瘤组织中的髓鞘结构及重要髓鞘蛋白。结果:电镜结果发现:听神经瘤组织中虽然缺失成熟的轴索结构但可以检测到早期的髓鞘样结构。免疫荧光研究和Western blotting研究结果显示:听神经瘤组织中检测不到轴索的标志性抗体——神经丝200的表达。并且启动髓鞘化的前哨信号及髓鞘致密化的重要组成蛋白——髓鞘碱性蛋白在听神经瘤组织中也无法检测到。而一种非成熟施万细胞的标志抗体——神经生长因子受体p75,在听神经瘤组织中可以检测到其表达。结论:在听神经瘤组织中的施万细胞失去了完成髓鞘化进程和重新形成致密的髓鞘并包绕轴索的能力,处于前髓鞘化施万细胞阶段。Objective：By detecting the myelin structure in acoustic tumor tissues,the cell origin and state of acoustic tumor tissues were investigated.Method：Immunofluorescence labeling,immunoblot analysis and electron microscopic study were performed to identify myelin structure and myelin protein in acoustic tumor tissues.Result：In this work,we found some early stage of myelin forming in acoustic tumor tissues,but there were no axon nor compact myelin formed and the myelin basic protein whose expression indicates the beginning of myelination was negative detected.We also found that the cell of acoustic tumor express p75,a marker for immature Schwann cells and mature non-myelin-forming Schwann cells.Conclusion：The date shown in this experiment indicates that the cell of acoustic tumor is in a premyelinating state.

关 键 词：施万细胞瘤 神经瘤 听 髓鞘 

分 类 号：R764.4[医药卫生—耳鼻咽喉科]