叶酸靶向超顺磁性氧化铁-多巴胺-叶酸纳米复合物的制备及体外MR成像  

Preparation of folate-mediated superparamagnetic iron oxide-dopamine-folate and its in vitro MR imaging

作　　者：王中领[1] 郭亮[1] 李勇刚[1] 陈铟铟[1] 顾宏伟[2] 鲁英杰[2] 

机构地区：[1]苏州大学附属第一医院放射科,江苏苏州215006 [2]苏州大学材料与化学化工学部,江苏苏州215021

出　　处：《中国医学影像技术》2011年第2期251-255,共5页Chinese Journal of Medical Imaging Technology

基　　金：江苏省自然科学基金(BK2006704-1);苏州市应用基础研究项目(YJS0917)

摘　　要：目的探讨叶酸靶向超顺磁性氧化铁-多巴胺-叶酸(NP-DPA-FA)纳米复合物对叶酸受体(FR)阳性肝癌细胞的靶向性及利用MRI对其进行监测的可行性。方法①制备NP-DPA-FA纳米复合物。②对Bel-7402细胞进行普鲁士蓝染色及体外竞争抑制试验。③将NP-DPA-FA复合物与Bel-7402人肝癌细胞及HL-7702正常人肝细胞孵育2 h后,进行普鲁士蓝染色及体外MR成像,观察其T2WI信号强度变化,并计算信号强度变化率。结果普鲁士蓝染色显示Bel-7402细胞内可见大量铁颗粒沉积,HL-7702细胞内铁颗粒沉积少见。Bel-7402细胞抑制竞争试验显示细胞内铁颗粒极少。铁浓度不同的NP-DPA-FA复合物分别与Bel-7402细胞及HL-7702细胞孵育后,MRI显示Bel-7402细胞的信号强度随NP-DPA-FA复合物铁浓度的升高而明显减低,HL-7702细胞信号强度减低相对不明显。结论叶酸介导的磁性纳米颗粒复合物对Bel-7402人肝癌细胞具有靶向性,应用MRI可对其进行监测。Objective To observe the targeting function of folate-conjugated superparamagnetic iron oxide-dopamine（NP-DPA-FA） to hepatoma cells via folate receptor（FR） and the feasibility for surveillance of tumor targeting with MRI.Methods ①At first,the preparation of NP-DPA-FA was completed.②Microscopic observation with Prussian blue staining and competitive inhibition was performed in Bel-7402 cells.③Bel-7402 cells and HL-7702 cells were incubated with NP-DPA-FA for 2 h,respectively.Prussian blue staining was performed to observe intracellular irons,while MRI was performed to study the signal intensity of cells.Results Much more intracellular irons were observed in Bel-7402 cells incubated with NP-DPA-FA than those in HL-7702 cells.Few intracellular irons were found in Bel-7402 cells by competitive inhibition.The signal intensity of Bel-7402 cells on T2WI decreased significantly with the exaltation of iron concentration in NP-DPA-FA,while no significant reduction of the signal intensity was found in HL-7702 cells.Conclusion NP-DPA-FA has good targeting ability to Bel-7402 cells in vitro.The cell targeting events could be monitored with MR imaging.

关 键 词：磁共振成像 靶向成像 叶酸 癌 肝细胞 

分 类 号：R445.2[医药卫生—影像医学与核医学] R735.7[医药卫生—诊断学]