微量量热法研究清开灵对金葡菌生长代谢的影响  

Microcalorimetric study on the metabolism of Staphylococcus aureus affected by Qingkailing injection

作　　者：金城 武彦舒[2] 张倩 李兴丰[4] 鄢丹 杜晓曦[5] 肖小河 

机构地区：[1]解放军第三O二医院全军中药研究所,北京100039 [2]陕西省榆林市第二医院,陕西榆林719000 [3]武警西藏总队医院,西藏拉萨850003 [4]湖南中医药大学药学院,湖南长沙410208 [5]国家食品药品监督管理局药品评价中心,北京100038

出　　处：《药学学报》2011年第2期193-197,共5页Acta Pharmaceutica Sinica

基　　金：国家“十一·五”科技支撑计划(2008BAI55B04);国家中医药管理局研究专项(06-07ZP58)

摘　　要：通过研究清开灵注射液对金葡菌生长代谢的影响,探索建立基于生物活性图谱的清开灵注射液的质量评价方法。本研究采用微量量热法,考察药物作用于金葡菌的生物热效应;对所测得的热输出曲线进行拟合,获得系列热动力学参数,对比分析各项热动力学参数及热输出曲线。结果显示,在一定范围内(0～5.00%),随清开灵剂量增加,金葡菌生长曲线呈"下降"趋势且指数生长期后延;其热动力学参数T1、T2、k1、P1、P2、I均与清开灵剂量呈现线性关系。清开灵注射液对金葡菌的半数抑菌量为3.26%,最佳抑菌量为5.47%;各类不同的清开灵注射液样品作用下的金葡菌生长曲线存在不同程度差异。实验结果表明,清开灵注射液对金葡菌生长具有较强的抑制作用,通过微量量热法研究,可对清开灵注射液的质量一致性进行初步评价。This paper is to report the investigation on the metabolic behavior of Staphylococcus aureus（S.aureus） after given Qingkailing injection,and with the aim of seeking for a new quality control method based on biological assessment.The growth thermogenic curves of S.aureus were determined by microcalorimetry and analyzed by computer.The results showed that in the concentration range of（0-5.00%）,the growth thermogenic curves of S.aureus were declined and removed back with increasing dosage of Qingkailing injection;the main parameters（T1,T2,k1,P1,P2 and I） and the dosage of Qingkailing injection have good correlation.The 50% inhibiting dosage is 3.26 %,and the optimal inhibiting dosage is 5.47%.Difference could be detected among the Qingkailing injection samples from different factories and different batches.It is proved that Qingkailing injection could inhibit the metabolic behavior of S.aureus,and microcalorimetry might be applied in the quality assessment of Qingkailing injection.

关 键 词：微量量热法 清开灵注射液 金葡菌 热输出曲线 

分 类 号：O642.3[理学—物理化学]