齐墩果酸在人血浆蛋白和血清白蛋白中结合率的测定  

Determination of protein binding rate of oleanolic acid in human plasma and serum albumin

作　　者：张弘[1] 张晖芬[1] 常会超[1] 韩笑[1] 毕开顺[1] 陈晓辉[1] 

机构地区：[1]沈阳药科大学药学院,辽宁沈阳110016

出　　处：《药学学报》2011年第2期243-246,共4页Acta Pharmaceutica Sinica

摘　　要：齐墩果酸（oleanolic acid,OA）又名庆四素（图1）,系五环三萜类化合物,以游离或结合成苷的形式广泛存在于白花蛇舌草、山楂、丁香、大枣、女贞子、枇杷叶、橡木和夏枯草等植物中。A LC-MS method was established for the determination of the protein binding rates of oleanolic acid in human plasma and serum albumin.The equilibrium dialysis combined with LC-MS to determine the total concentration in plasma and free drug concentration of oleanolic acid was carried out.The human plasma protein binding rates of oleanolic acid at three concentrations were 79.6%,81.9% and 63.3%,respectively.The human serum albumin protein binding rates of oleanolic acid at three concentrations were 53.5%,56.6% and 47.7%,respectively.The method is shown to be simple,accurate,sensitive and specific for the determination of biological samples.The protein binding rates in human plasma and serum albumin were of high strength.

关 键 词：齐墩果酸 蛋白结合率 LC-MS 平衡透析法 

分 类 号：R917[医药卫生—药物分析学]