TGF-β_1基因多态性与乙型肝炎肝硬化的相关性研究  

Association between TGF-β_1 Gene Polymorphism and Hepatocirrhosis in Chinese Patients with Chronic HBV Infection

作　　者：石伟珍[1] 过建春[1] 陈群伟[1] 王宇芳[1] 荀运浩[1] 施军平[1] 娄国强[1] 

机构地区：[1]浙江中医药大学附属杭州第六医院,杭州310014

出　　处：《医学研究杂志》2011年第1期93-96,共4页Journal of Medical Research

基　　金：浙江省自然科学基金资助项目(Y205410)

摘　　要：目的探讨转化生长因子-β1(TGF-β1)基因多态性是否与慢性HBV感染人群肝硬化发生的遗传易感相关。方法收集173例中国汉族慢性HBV感染患者(肝硬化组42例和非肝硬化组131例)和52例健康对照者的外周血标本,提取白细胞DNA,基因直接测序法检测TGF-β1基因4个多态性(869T/C、915G/C、-800 G/A、-509 C/T),ELISA法检测TGF-β1血清浓度,比较各组TGF-β1基因型分布频率、等位基因频率及TGF-β1血清水平的差异。结果 (1)乙型肝炎肝硬化组TGF-β1869位点以TT基因型为主(47.6%),高于非肝硬化组(25.2%)和对照组(19.2%),差异有统计学意义(P<0.05);869T等位基因频率(63.1%)明显高于正常对照组(44.2%),差异有统计学意义(P<0.05),乙型肝炎肝硬化组与慢性HBV感染非肝硬化组(53.8%)、慢性HBV感染非肝硬化组与正常组869T等位基因携带率相比,差异无统计学意义(P>0.05)。(2)所检其余多态性位点基因型及基因频率未见组间统计学差异。(3)869T/C不同基因型患者的血清TGF-β1水平不一,T/T型携带者较T/C和C/C型可见明显增高(P<0.017)。结论 TGF-β1869T可能与汉族人群乙型肝炎肝硬化的遗传易感相关,其机制可能与该多态性对TGF-β1血清水平的影响有关。Objective To investigate the association between transforming growth factor beta1（TGF-β1） gene polymorphism and liver cirrhosis with chronic HBV infection in Chinese Han population.Methods Blood samples were obtained from 173 HBV infectors in Chinese Han population（including 131 non-hepatocirrhosis cases and 42 hepatocirrhosis cases） and 52 healthy controls.The 4 variances of TGF-β1 genes（TGF-β1 869T/C,915G/C,-800 G/A,-509 C/T） were identified by DNA sequencing,while TGF-β1 level in serum was also measured by ELISA.Results（1）The frequency of TGF-β1 869TT genotype in chronic HBV hepatocirrhosis patients（47.6%） was higher than that in non-hepatocirrhosis cases（25.2%） and controls（19.2%） significantly,respectively.The allele frequency of TGF-β1 869T in hepatocirrhosis patients（63.1%） was significant higher than that in controls（44.2%）（P0.05）.（2）There was no significant difference within groups relating to the other gene-loci of TGF-β1（P0.05）.（3）In the test of TGF-β1 serum level within 869T/C different genotype,it was found that serum level of TGF-β1 in T/T was increased comparing to T/C and C/C genotypes,and the difference was statistically significant（P0.017）.Conclusion There may be an association between TGF-β1 869T and Chinese Han inherited susceptibility of liver fibrosis in chronic HBV infectors,which influences the serum level of TGF-β1 probably,then participates in the development of liver fibrosis.

关 键 词：转化生长因子-Β1 肝炎病毒 乙型 肝硬化 基因 多态现象(遗传学) 

分 类 号：R392.12[医药卫生—免疫学]