来曲唑治疗大鼠子宫内膜异位症模型作用及机制的研究  

Effect and mechanism of letrozole treating rat endometriosis model

作　　者：宋淑芳[1] 尹利荣[1] 齐之迎[1] 

机构地区：[1]天津医科大学第二医院妇科,天津300211

出　　处：《现代妇产科进展》2011年第1期35-38,共4页Progress in Obstetrics and Gynecology

摘　　要：目的:探讨第3代芳香化酶抑制剂(aromatase inhibitors,AIs)来曲唑治疗大鼠子宫内膜异位症模型的效果及作用机制。方法:用自体子宫内膜移植方法建立大鼠内异症模型,随机将20只建模成功大鼠分为来曲唑组和盐水对照组,比较治疗前后EM大鼠异位病灶体积的变化;免疫组化法检测异位内膜病灶中细胞色素P450芳香化酶(P450arom)、环氧合酶-2(COX-2)、血管内皮生长因子(VEGF)、增殖细胞核抗原(PCNA)蛋白的表达;末端转移酶介导的缺口末端标记法(TUNEL)检测细胞凋亡情况;放射免疫分析法测定大鼠血清FSH、LH、E2水平。结果:来曲唑组异位病灶体积缩小(P<0.01);异位内膜中P450arom、COX-2、VEGF、PCNA蛋白表达与对照组相比均明显降低(P<0.05);来曲唑组异位内膜细胞凋亡率较对照组明显增加(P<0.01);两组大鼠血清FSH、LH、E2水平无明显差异(P>0.05)。结论:来曲唑治疗大鼠子宫内膜异位症模型有效。来曲唑通过降低异位内膜局部P450arom、COX-2、VEGF蛋白表达及抑制异位内膜增殖并促进凋亡发挥治疗子宫内膜异位症的作用。来曲唑对大鼠血清FSH、LH、E2水平无明显影响。ObjectiveTo explore the effect and mechanism of letrozole treating endometriosis（EM）rat models.Methods：Surgically transplanted autologous uterine tissues in ectopic site beside the uterines in rats were used as animal models.Twenty EM rat models were randomly divided into letrozole group and control group.The change of ectopic lesion volume in each group was compared before and after the treatment.Immunohistochemistry was used to detect the expression of P450arom,COX2,VEGF and PCNA in the endometriotic tissues in the rat models;Apoptotic cells were assessed by the terminal deoxynucleotidyl transferase-mediated deoxy-UTP nick-end labeling（TUNEL） assay.The serum levels of FSH,LH and E2 were tested by radioimmunoassay.Results：The volumes of the endometriotic tissues in letrozole group were reduced compared with the control group（P0.05）;expressions of P450arom,COX2,VEGF and PCNA proteins decreased markedly in tissues of EM in rat models after letrozole therapy（P0.01）,the apoptotic rates increased clearly in letrozole group than those in control group（P0.01）;there were no significant difference in the serum levels of FSH,LH and E2 between the two groups（P0.05）.Conclusion：Letrozole plays a role in treating EM by means of decreasing the expressions of P450arom,COX2 and VEGF proteins,and increasing the apoptosis while decreasing the proliferation of the ectopic tissues.Letrozole has no effect on the serum levels of FSH,LH and E2.

关 键 词：来曲唑 动物疾病模型 大鼠 Sprague-Dawley 子宫内膜异位症 芳香化酶 

分 类 号：R711.71[医药卫生—妇产科学]