DNA barcoding of 18 species of Bovidae  

作　　者：CAI YanSen ZHANG Liang SHEN FuJun ZHANG WenPing HOU Rong YUE BiSong LI Jing ZHANG ZhiHe 

机构地区：[1]Department of Medical Biology and Genetics, Luzhou Medical College, Luzhou 646000, China [2]Key Laboratory for Conservation Biology on Endangered Wildlife of Siehuan Province, Chengdu Research Base of Giant Panda Breeding, Chengdu 610000, China [3]Key Laboratory of Conservation Biology on Endangered Wildlife of Sichuan Province, College of Life Sciences, Sichuan University, Chengdu 610000, China

出　　处：《Chinese Science Bulletin》2011年第2期164-168,共5页

基　　金：supported by the grant from Chengdu Giant Panda Breeding Research Foundation(CPF08004)

摘　　要：Genetic divergences of mitochondrial cytochrome c oxidase subunit I genes,known as DNA barcodes,have been used in species identification in the animal kingdom.Barcodes can assist field workers and taxonomists to determine groups in need of taxa analysis,and facilitate the recognition of appropriate populations and scales for conservation planning.In this study,18 species of Bovidae were selected to evaluate the effectiveness of DNA barcoding for species differentiation.The results showed that all but 2 species had unique DNA barcodes.The mean intraspecific variation was 0.63%,yielding a threshold of 6.3% for flagging putative species.The results supported the inference that barcode variation within species of mammals is somewhat higher than within other animal groups.The present study validated the effectiveness of barcoding for the identification of bovid species.Genetic divergences of mitochondrial cytochrome c oxidase subunit I genes, known as DNA barcodes, have been used in species identification in the animal kingdom. Barcodes can assist field workers and taxonomists to determine groups in need of taxa analysis, and facilitate the recognition of appropriate populations and scales for conservation planning. In this study, 18 species of Bovidae were selected to evaluate the effectiveness of DNA barcoding for species differentiation. The results showed that all but 2 species had unique DNA barcodes. The mean intraspecific variation was 0.63%, yielding a threshold of 6.3% for flagging puta- tive species. The results supported the inference that barcode variation within species of mammals is somewhat higher than within other animal groups. The present study validated the effectiveness of barcoding for the identification of bovid species.

关 键 词：条码识别 DNA 牛科 细胞色素C氧化酶 物种鉴定 遗传差异 生物分类 保护规划 

分 类 号：Q523[生物学—生物化学] TP391[自动化与计算机技术—计算机应用技术]