成骨细胞的体外剪切应力加载实验的变形分析及信号转导区域探讨  

Study on cellular deformation of in vitro osteoblasts＇ shear stress loading experiment and their mechanotransduction region

作　　者：滕维中[1] 张西正[2] 

机构地区：[1]广西大学物理科学与工程技术学院,南宁530005 [2]军事医学科学院卫生装备研究所,天津300161

出　　处：《国际生物医学工程杂志》2010年第6期340-342,共3页International Journal of Biomedical Engineering

基　　金：国家重点基金资助项目（10832012）

摘　　要：目的 根据已有体外培养鼠成骨细胞的参数实验数据,估算剪切应力加载实验中细胞整体剪切形变,借以研究细胞的主要转导区域.方法 计算过程采用黏弹性力学理论,对细胞运用了标准黏弹性模型,并简化其膜所受剪切力为均匀.结果 细胞剪切力产生的细胞变形大约是引起成骨细胞相同生物学响应的拉伸加载变形的十分之一.结论 从细胞总的力学刺激生物学响应来看,剪切应力加载实验中细胞的整体变形所产生的力学转导是可以忽略的,主要转导区域在承受剪切应力的细胞膜.Objective To study the main mechanotransduction area and to estimate the overall shear deformation of rat osteoblasts in shear stress loading experiment based on data acquired from the in vitro rat osteoblast experimental, and to study the effects of four-point bending medium flow generated shear stress on cells. Methods Viscoelastic mechanics theory was used in the calculation process, the standard viscoelastic model was adopted for cells, and shear force on the cellular surface was simplified to be uniform. Results The cellular deformation caused by shear force was about one-tenth of that from tensile loading experiment which induced equivalent biological response. Conclusion In terms of mechanical stimulus induced biological responds, the mechanical transduction caused by cellular deformation in shear stress loading experiment is negligible, and the main transduction area is in the cellular membranes experiencing shear stress.

关 键 词：成骨细胞 剪切应力 细胞变形 力学转导 

分 类 号：R6[医药卫生—外科学] R3[医药卫生—临床医学]