-80℃冰冻保存对血小板聚集功能的影响  

Effect of the Platelet Aggregation Function after Cryopreservation at -80℃

作　　者：何丽娟[1] 邓为彬 李永萍[1] 

机构地区：[1]大理学院附属医院,云南大理671000 [2]嘉兴市第二医院,浙江嘉兴314000

出　　处：《大理学院学报（综合版）》2010年第12期35-37,共3页Journal of Dali University

基　　金：大理学院科研基金资助项目(2007X11)

摘　　要：目的:初步了解-80℃冰冻保存对血小板聚集功能的影响。方法:采集健康人新鲜血进行离心,制作富含血小板的血浆(PRP),用血小板聚集仪检测聚集功能;将分离的血小板分别加入保护剂5%二甲基亚砜(5%DMSO)后迅速置于-80℃低温冰箱保存1月,复苏后再次对血小板聚集功能进行检测。结果:血小板聚集率和聚集率单位时间的变化冰冻前后对比均无统计学意义(P>0.05)。结论:血小板加终浓度为5%DMSO,在-80℃条件下冰冻保存1个月,血小板的聚集功能稳定。） Objective： A preliminary study of the effect of platelet aggregation function cryopreservation at -80℃.Methods： Fresh blood were obtained from the health donors,platelet-rich plasma（PRP） were prepared after centrifugation.Platelet aggregation was measured by platelet aggregation machine.Then,the platelet samples were cold preserved at -80℃ after added with protective agent DMSO（5%DMSO）.One month later,the platelet aggregation was measured again after rapid recovery.Results： The platelet aggregation rate（PAG） and the change of aggregation rate per unit time are not statistically significant difference（P0.05） before and after the cold preservation.Conclusion： After cold preservation under the condition of 5% DMSO,-80℃ for 1 month,platelet aggregation function retained stability.

关 键 词：血小板 冰冻保存 聚集功能 

分 类 号：R457[医药卫生—治疗学]