红掌花叶病病原鉴定及检测方法的建立  

Development of a RT-PCR based method for identification of Anthurium andraeanum mosaic virus pathogen

作　　者：刘福秀[1] 林明光[1] 潘英文[1] 王安石 

机构地区：[1]海南出入境检验检疫局热带植物隔离检疫中心,海口570311

出　　处：《广西农业科学》2010年第12期1296-1298,共3页Guangxi Agricultural Sciences

基　　金：国家科技支撑计划项目(2007BAD45B07);海南省重大研发专项项目(080201)

摘　　要：以红掌为材料,利用RT-PCR方法鉴定出红掌花叶病的一种病原——芋花叶病毒(Dasheen mosaic virus,DMV),并建立了该病毒的RT-PCR检测方法。依据该方法,将从0.1 g染病组织中提取的总RNA稀释500倍,仍能从中检测出DMV的存在,说明该方法的灵敏度高。该检测方法的建立,为红掌栽培,尤其是工厂化生产红掌无毒种苗提供了技术支持。The present research work has been undertaken to identify the pathogen （Dasheen mosaic virus, DMV） of Anthurium andraeanum rrlosaic virus using RT-PCR method, and to develop a reliable RT-PCR protocol for its detection, The method developed can detect the DMV pathogen in 500× diluted total RNA extracted from 0.1 g diseased tissues, which indicated very high sensitivity of this method for DMV detection. The establishment of DMV detection method will be helpful in commercial production of Anthurium andraeanum virus-free seedings.

关 键 词：红掌 芋花叶病毒 鉴定 检测方法 

分 类 号：S436.8[农业科学—农业昆虫与害虫防治]