广西鹅α干扰素基因的克隆与序列分析  

Cloning and sequence analysis of IFN-α gene in Guangxi goose

作　　者：付薇[1,2] 蒋家霞[1] 孙翔翔[1] 易春华[1] 何奇松[1] 马琳[2] 徐贤坤[2] 黄胜斌[2] 熊毅[2] 

机构地区：[1]广西大学动物科学技术学院,南宁530005 [2]广西动物疫病预防控制中心,南宁530001

出　　处：《广西农业科学》2010年第12期1335-1338,共4页Guangxi Agricultural Sciences

基　　金：广西科技攻关项目(桂科攻0719004-3G)

摘　　要：根据GenBank上已发表的东北白鹅α干扰素(IFN-α)基因(AY524422)序列设计1对特异性引物,采用RT-PCR从经鹅副粘病毒(GPMV)刺激培养的鹅外周血淋巴细胞中克隆获得广西鹅IFN-α基因,将其连接pMD18-T载体、转化DH5α感受态细胞后进行序列分析。结果表明,广西鹅IFN-α基因完整的开放阅读框大小为576 bp,共编码192个氨基酸残基,其推导的氨基酸中有7个半胱氨酸残基和2个潜在的糖基化结合位点(NDT和NHT)。与GenBank上已公布的其他IFN-α基因进行同源性比较分析,发现广西鹅IFN-α基因与东北白鹅的同源性最高(核苷酸同源性为98.3%、氨基酸同源性为96.4%),与鸡、牛、猪、鼠、犬、人等的同源性较低。说明IFN具有种属特异性,且亲缘关系越近同源性越高,这与物种之间的进化亲缘关系一致。A pair of primer was designed using the nucleotide sequence of Northeast white goose IFN-α gene obtained from GenBank （Accession No. AY524422）. Total RNA was extraαed from cultures of goose peripheral blood lymphocytes under stimulation of GPMV, and the cDNA was amplified using reverse transcription-polymerase chain reaαion （RT-PCR）, which was subsequently cloned into pMD18-T veαor and sequenced. The results showed that the open reading frame （ORF） of Guangxi goose interferon-α （GoIFN-α） gene consisted of 576 bp, encoding 192 amino acid residues. Among them, there were seven cysteine residues and two potential glycosyl binding site （NDT and NHT）. Compared with the sequences of other goose IFN-α gene in GenBank, the homologies of nucleotide and amino acid bαween Guangxi goose and white Northeast goose （AY524422） was found the highest （98.3 and 96.4%, respectively）. However, these two indices among Guangxi goose and chicken, cow, swine, rat, dog and human, αc. were lower. These results indicated that the IFN had species specificity, the homologies of interferon among different animal species depend on their genαic relationships.

关 键 词：鹅 Α干扰素 基因克隆 序列分析 广西 

分 类 号：S858.33[农业科学—临床兽医学]