血红素氧化酶-1基因启动子区(GT)n重复序列多态性与原发性高血压及冠心病易感性的关联研究  

Association Study of (GT)n Polymorphism in Hemeoxygenase-1 Gene Promoter and Susceptibility of Primary Hypertension and Coronary Artery Disease

作　　者：张国红[1] 麦瑞琴 陈宋明 

机构地区：[1]汕头大学医学院病理学教研室,广东汕头515041 [2]第一附属医院心血管内科,广东汕头515041

出　　处：《汕头大学医学院学报》2010年第4期202-204,共3页Journal of Shantou University Medical College

基　　金：广东省自然科学基金资助项目(07008215)

摘　　要：目的:探讨HO-1基因启动子区(GT)n重复序列多态性与原发性高血压、冠心病以及原发性高血压合并冠心病易感性间的关联。方法:采用荧光标记PCR以及毛细管电泳相结合技术,检测HO-1基因启动子区(GT)n重复序列多态性在91例原发性高血压、111例冠心病、189例原发性高血压合并冠心病患者,以及性别、年龄相匹配的91例对照个体中等位基因和基因型频率分布差异。多因素降维法分析基因型与吸烟、糖尿病、高血脂等危险因素间的交互作用。结果:与对照组比,等位基因频率仅在冠心病组中分布有统计学意义(P<0.05),SL+LL基因型频率在原发性高血压(P<0.05)和冠心病组(P<0.01)中有统计学意义(P<0.05),而在原发性高血压合并冠心病组中无统计学意义。SL+LL基因型与吸烟交互作用增加了原发性高血压和冠心病的发病风险,而对于原发性高血压合并冠心病发病风险无影响。结论:HO-1基因启动子区(GT)n重复序列多态性可作为原发性高血压、冠心病发病风险评估的分子遗传标记。Objective：To assess the association between the length of（GT）n repeats in the HO-1 gene promoter and the susceptibility for patients of primary hypertension,coronary artery disease,primary hypertension with coronary artery disease.Methods：Amplification and labeling of PCR products for capillary electrophoresis were performed to genotype.Ninety-one patients with primary hypertension,111 patients with coronary artery disease,189 patients of primary hypertension with coronary artery disease and 91 control subjects with sex and age matching were collected.The multifactor dimensionality reduction method was conduct to detect interactions with genotypes and smoking,diabetes and heperlipidemia. Results：Allelic frequency did differ significantly between coronary artery disease patients and control subjects;Genotypic frequencies were different in primary hypertension（P0.05）,coronary artery disease（P0.01）and control groups.There were interaction between SL＋LL genotype and smoking for risk of primary hypertension（OR：2.7）and coronary artery disease（OR：3.1）.However,there were no significant effects of polymorphism with the susceptibility of primary hypertension with coronary artery disease.Conclusion：Longer（GT）n repeat allele in HO-1 promoter is a susceptibility marker of primary hypertension with coronary artery disease.

关 键 词：高血压 冠心病 血红素氧化酶-1 易感性 

分 类 号：R544.1[医药卫生—心血管疾病]