胸膜肺炎放线杆菌血清8型自转运黏附素基因克隆测序及功能区对比  

Cloning,Sequencing and Functional Comparison of APP Serotype 8 Actinobacillus pleuropneumoniae Adhesins

作　　者：王瑜[1] 雷连成[1] 韩文瑜[1] 谢芳[1] 李林溪[1] 周靓[1] 杨舒心[1] 胡本钢[1] 邢艳苹[1] 计群[1] 路荣[1] 刘晓贺[1] 何伯萍[1] 

机构地区：[1]吉林大学畜牧兽医学院,吉林长春130062

出　　处：《河北科技师范学院学报》2010年第4期40-46,共7页Journal of Hebei Normal University of Science & Technology

基　　金：国家自然科学基金项目(项目编号:31072133);吉林大学高校基本科研项目(项目编号:421021356201)

摘　　要：为研究胸膜肺炎放线杆菌(APP)三聚体自转运黏附素(TAAs)的功能,通过PCR的方法首次获得APP血清8型的基因序列并提交GenBank(bankit1392817 HQ399552),运用生物信息学的方法对APP血清8型基因序列进行分析,并与GenBank公布的已知血清型的基因序列进行比对。结果表明:与血清7型同源性达到93%;与血清3,5b型同源性均达到92%。对APP血清8型序列软件预测分析发现,含有大量的Ylhead和Hep_Hag基序,所得序列是三聚体自转运黏附素N段序列,具有良好的抗原性。In order to study the function of Actinobacillus pleuropneumoniae（APP）trimeric autotransporter adhesins（TAAs）,PCR was first used to obtain the gene sequence of the serotype 8,which was then submitted to GenBank（bankit1392817 HQ399552）and compared with the genes in the Genbank.The results showed that the gene similarities between serotype 8 and serotype 7,serotype 3 and 5b were 93%,92%,92% respectively.The sequence was the trimeric autotransporter adhesins（TAAs）N-terminal end,containing a great quantity of Ylhead and Hep_Hag motifs.The sequence was also found to have good antigenicity.This result provides an important basis for research the colonization motif and the virulence factors of Actinobacillus pleuropneumoniae.

关 键 词：胸膜肺炎放线杆菌 三聚体自转运黏附素 PCR APP 

分 类 号：S852.619[农业科学—基础兽医学]