Establishment of Plant Regeneration System from Immature Embryos of Maize(Zea mays L.) Inbred Lines  

玉米自交系幼胚再生体系建立的研究(英文)

作　　者：秦新民[1] 曾德龙[1] 覃屏生[1] 

机构地区：[1]广西师范大学生命科学学院,广西桂林541004

出　　处：《Agricultural Science & Technology》2010年第8期61-63,共3页农业科学与技术（英文版）

基　　金：Supported by Natural Science Foundation of Guangxi Province(Guikezi 0991096)~~

摘　　要：[Objective] The aim was to explore the conditions of high frequency induction of embryonic callus and plant regeneration of maize. [Method] Immature embryos of maize inbred lines were used as explants to study the effect of different 2,4-D concentrations on the induction of callus,the effect of different 6-BA concentrations on the differentiation of test-tube plantlet,as well as the effect of different IBA concentrations on the rooting of test-tube plantlet. [Result] 2,4-D showed obvious effect on the induction of inducement rate of maize,and the optimum induction medium was：N6 ＋ 2 mg/L of 2,4-D ＋ 500 mg/L of CH ＋ 500 mg/L of Pro ＋10 mg/L of AgNO3; the optimum differentiation medium was：N6 ＋ 0.5 mg/L of BA ＋ 500 mg/L of Pro; the optimum for the rooting of test-tube plantlet was 1/2 MS ＋ 0.5 mg/L of IBA. [Conclusion] The study had provided basis for the genetic transformation of maize.以玉米自交系幼胚为外植体,研究了不同2,4-D浓度对愈伤组织诱导、BA浓度对苗分化和IBA对试管苗生根的影响。结果表明:2,4-D对胚性率诱导有明显影响,其诱导最佳培养基为N6+2,4-D2mg/L+水解酪蛋白(CH)500mg/L+脯氨酸(Pro)500mg/L+AgNO310mg/L;苗分化适宜的培养基是N6+BA0.5mg/L+水解酪蛋白500mg/L;试管苗生根的最佳培养基为1/2MS+IBA0.5mg/L。

关 键 词：MAIZE Immature embryo CALLUS Tissue culture Plant regeneration 

分 类 号：S513[农业科学—作物学]