Research on Genetic Diversities between Xiaogan Water Chestnut and Wild Chestnut by Randomly Amplified Polymorphic DNA Technology  

RAPD法研究孝感荸荠和野生荸荠的遗传差异性(摘要)(英文)

作　　者：盛继群[1] 曹姣[1] 李建华[1] 

机构地区：[1]孝感学院生命科学技术学院湖北省作物病害监测和安全控制重点实验室,湖北孝感432000

出　　处：《Agricultural Science & Technology》2010年第8期84-86,90,共4页农业科学与技术（英文版）

基　　金：Supported by Natural Science Foundation of Hubei Province(2005ABA084);Major Projects of Hubei Provincial Department of Education (04Z002)~~

摘　　要：[Objective] The aim was to study the genetic diversities between Xiaogan water chestnut and wild chestnut with randomly amplified polymorphic DNA （RAPD） technology. [Method] Genetic diversities of the local cultivated water chestnut,wild chestnut,Lepironia articulata and Scirpus planiculmis Fr. Schmidt were analyzed by RAPD technology. [Result] Among the screened random primers 841,842,807 and 840,the polymorphism of amplification product of 841 was evident,and the obtained bands in electrophoresis were clear and showed good repeatability. Cluster analysis result showed that the affinity of cultivated water chestnut and wild water chestnut was nearer than that between Lepironia articulata and Scirpus planiculmis. [Conclusion] The research provides theoretical basis for cultivating high-quality new varieties of water chestnut.[目的]采用RAPD法研究孝感荸荠和野生荸荠的遗传差异性。[方法]利用随机扩增多态性DNA(RAPD)技术,对地方栽培种孝感荸荠、野生荸荠、蒲草和扁杆藨草4种植物的基因组DNA进行了遗传差异性分析。[结果]筛选出841、842、807和840共4个随机引物,其中841随机引物的扩增产物多态性明显,获得清晰重复性好的条带。聚类结果显示栽培荸荠和野生荸荠的亲缘性相对于蒲草和鹿茸杆藨草而言较近。[结论]该研究为培植优质的荸荠新品种提供了理论基础。

关 键 词：Water chestnut RAPD Cluster analysis Genetic diversities 

分 类 号：S645.3[农业科学—蔬菜学]